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Somatic embryogemic regeneration of Acacia mangium

A technique for somatic embryos and explants, used in, preparing somatic embryos of Acacia mangium, regenerating plants or regenerating bodies, amang, regenerating Acacia mangium by somatic embryogenesis, sand-soil mixture, cloning and planting stock, mature culture Continue to cultivate on the base and germination medium, and grow into the field of embryogenic tissue, which can solve the problems of unstable plant tissue and abnormal type.

Inactive Publication Date: 2006-01-04
TEMASEK LIFE SCIENCES LABORATORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This means that the regenerant is less likely to be of chimeric origin, because if the regenerant is derived from groups of cells rather than single cells, the plant tissue may be chimeric or unstable and produce abnormal

Method used

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  • Somatic embryogemic regeneration of Acacia mangium
  • Somatic embryogemic regeneration of Acacia mangium
  • Somatic embryogemic regeneration of Acacia mangium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Reagents and media

[0026] name

molecular formula

concentration

(mg / l)

Sigma

catalog number

Macronutrients

ammonium nitrate

NH 4 NO 3

1650

A-3795

potassium nitrate

KNO 3

1900

P-8291

Calcium Chloride Dihydrate

CaCl 2 .2H 2 o

440

C-2536

Magnesium Sulfate Heptahydrate

MgSO 4 .7H 2 o

370

M-7774

Potassium dihydrogen phosphate anhydrous

K H 2 PO 4

170

P-8416

Ferrous Sulfate Heptahydrate

FeSO4.7H2O

27.8

F-8263

Ethylenediaminetetraacetic acid

(EDTA)

C 10 h 14 N 2 o 8 Na 2 .2H 2 o

(Na 2 EDTA)

37.3

E-6635

Micronutrients

potassium iodide

KI

0.83

P-8166

boric acid

h 3 BO 3

6.2

B-9645

Manganese Sulfate Monohydrate

MnSO 4 .H 2 o

6.9

M-7899

...

Embodiment 2

[0057] Example 2: Plant material

[0058] 1.5-month-old immature green pods were collected from 20-meter-tall mature Acacia mangium Willd trees along Science Park Avenue, Singapore. Gently separate the green seeds from the pods and rinse with sterile double deionized water (ddH 2 O) wash 4-5 times. The surface of the green seeds was disinfected with 30% commercially available sodium hypochlorite bleach (NaClO) for 5 min and washed with sterile ddHO 2 After washing 5 times with O, the seeds were washed with 0.1% HgCl 2 Sterilize for 6 minutes, and use sterile ddH 2 O wash 5 times. The seed coat is removed with sterile forceps, and the embryos are isolated. Hypocotyls (EA) (Fig. 1A) were gently detached with sterile forceps and used as explants for embryogenic callus induction.

Embodiment 3

[0059] Embodiment 3: culture condition

[0060] After autoclaving at 121°C for 25 minutes, all media were adjusted to pH 5.8. The plant growth regulators tested in the experiment were added to the medium after autoclaving, but other substances were added to the medium before autoclaving. All cultures were maintained at 28°C at a luminance of 23.41-26.01 μmol.s -1 m -2 Range of warm white fluorescent lamps with a photoperiod of 16 hours.

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Abstract

The present invention relates to the field of somatic embryo production, particularly to methods of the regeneration of Acacia mangium through somatic embryogenesis. More specifically, the present invention relates to a method for regeneration of plants of Acacia mangium by culturing explants of immature zygotic embryos on callus induction medium to grow embryogenic tissue. Culturing of the embryogenic tissue is continued on somatic embryo maturation medium and germination medium. The germinated embryos are further converted on acclimatized plants for field planting. The method is well suited for producing clonal planting stock useful for reforestation.

Description

Background of the invention [0001] The invention relates to the field of somatic embryo production, in particular to a method for regenerating Acacia mangium through somatic embryogenesis. More particularly, the present invention relates to a method for growing embryogenic tissue by culturing explants of immature zygotic embryos on a callus induction medium. Embryogenic tissue was continued on somatic embryo maturation medium and germination medium. The germinated embryos are further converted into domesticated plants for planting in the field. The invention is particularly suitable for generating clonal planting stocks for reforestation. [0002] The publications and other material cited herein to illustrate the background of the invention or to provide additional illustration of practical application are hereby incorporated by reference and are each collectively listed in the accompanying Reference List. [0003] Forests are very important to the world economy and to main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/00A01H4/00C12N5/02
CPCA01H4/005A01H4/008C12N5/0025Y02P60/40Y02A40/22
Inventor 谢德玉洪焰
Owner TEMASEK LIFE SCIENCES LABORATORY