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Method for preparing tissue engineered corneal epithelium implantation sheet from epidermis stem cell, and its application

An epidermal stem cell and artificial cornea technology, applied in the field of tissue engineering, can solve problems such as corneal limbal stem cell transplantation, and achieve the effects of weakening immune memory, good transparency and strong operability

Inactive Publication Date: 2006-02-15
陕西九州生物医药科技集团有限公司
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method of using epidermal stem cells instead of limbal stem cells to construct tissue-engineered corneal epithelium and its use in reconstructing the defected ocular surface of limbal stem cells, so as to solve the problem of clinically inability to perform autologous cornea due to damage to both eyes. Limbal stem cell transplantation is a successful method to construct tissue-engineered corneal epithelium by using epidermal stem cells to treat limbal stem cell defects

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  • Method for preparing tissue engineered corneal epithelium implantation sheet from epidermis stem cell, and its application
  • Method for preparing tissue engineered corneal epithelium implantation sheet from epidermis stem cell, and its application
  • Method for preparing tissue engineered corneal epithelium implantation sheet from epidermis stem cell, and its application

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Embodiment Construction

[0028] The content of the invention will be further described below in conjunction with the Guanzhong dairy goat as the experimental model.

[0029]1. Production of model animals: select 3-4 year old healthy adult Guanzhong dairy goats without eye diseases, intramuscularly inject 2.4ml-2.6ml of veterinary 846 mixture for general anesthesia, and disinfect around the eyes with iodine. Lying sideways on Baoding, laying surgical drapes, eyelid opening with eyelid speculum, double antibiotic saline (penicillin sulfate 2×10 5 u / L, streptomycin 200mg / L) to flush the ocular surface and conjunctival sac, 5ml each of 2% lidocaine hydrochloride and 1.3% bupivacaine hydrochloride, and retrobulbar anesthesia. Under the ophthalmic operating microscope, cut the bulbar conjunctiva and subconjunctival fascia circularly along the limbus of the operated eye to expose the sclera. The sclera 1mm outside the limbus and the remaining conjunctiva, the limbus and the corneal epithelial tissue 2mm ins...

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Abstract

This invention discloses the preparation for artificial corneal epithelium implants made up of skin dry cells and a usage in transplanting and repairing a blind eye skinsbecause of defecting limbus corneae dry cells. The artificial corneal epithelium implant applies skins of adult neck or milk goat ear edge in asepsis to purify skin dry cells with TC and clone selection, amplification with serum-free medium and detect the got dry cells with CK19, P63, integrin-beta1, label protein immune tissue then to vaccinate the purified dry cells on to human amnion frame of depithelial cell nurtured by serum-free medium for 21days. The cells are separated on the amnion to form stratified layers and become cell amnion implants. The cells are divided into 5-6 layers having the structure of normal cornea epithelia.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and in particular relates to a method for preparing a tissue-engineered corneal epithelial graft constructed from skin-derived epidermal stem cells as seed cells, and using the epidermal stem cells to construct a tissue-engineered corneal epithelial graft Use of transplantation to repair ocular surface blind due to limbal stem cell defect. Background technique [0002] The normal ocular surface is covered with highly differentiated corneal and conjunctival epithelium, which are composed of two types of epithelial cells that are morphologically distinct. The conjunctival epithelium is composed of mucin-secreting goblet cells and is highly vascularized. The cornea, composed of unkeratinized squamous epithelium, maintains the integrity of the ocular surface and normal vision. Corneal epithelial cells are derived from corneal stem cells of the limbus [Sche...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/08A61F2/14
Inventor 杨学义屈雷王馨窦忠英
Owner 陕西九州生物医药科技集团有限公司
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