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Triazophos pesticide residual degrading strain and strain therefrom

A technology for the degradation of pesticide residues and bacterial agents, which is applied in the field of high biotechnology, can solve the problems of narrow degradation spectrum of bacterial strains and use restrictions, and achieve the effects of low production and use costs, increased added value, and good removal effect

Inactive Publication Date: 2006-05-24
南京农大环境生物工程有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

In the prior art, our research group already has an invention patent for degrading bacteria (ZL96106317.3, publication number: CN1145408A, publication date: March 19, 1997), which is a strain of Bacillus J5, which can effectively degrade methylamine Phosphorus, but the strain has a narrow degradation spectrum and can only degrade one pesticide, methamidophos, so its use is limited

Method used

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  • Triazophos pesticide residual degrading strain and strain therefrom
  • Triazophos pesticide residual degrading strain and strain therefrom
  • Triazophos pesticide residual degrading strain and strain therefrom

Examples

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Embodiment Construction

[0016] Examples of the present invention are described below.

[0017] Activate the original seed of the microbial organophosphorus pesticide residue-degrading bacteria MP-4 of the present invention on a petri dish, measure its degradation performance, and inoculate it on the inclined surface of a test tube for later use. The test tube seeds were inoculated into 1000ml shake flasks containing 200ml broth medium, cultured with constant temperature shaking until logarithmic phase, and prepared to inoculate seed tanks. 500 liters of seed tanks, 400 liters of feeding capacity, the medium formula is: glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl0.01%, CaCO 3 0.3%, yeast extract 0.02%, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure damp heat sterilization, after cooling to 33°C, inoculate the above-mentioned cultivated shake flask strains into a 500-liter seed tank according to the inoculum amount of 10%, and cultivate to the logarithmic...

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Abstract

The invention provides a triazophos pesticide residue degrading bacterium and the bacterial agent produced therefrom. The used bacterial strain is Gram staining negative bacterial strain MP-4, which is identified as Ochrobactrum.sp. The main biological characteristic is G - , the bacteria are rod-shaped, about 1.58×0.79μm in size, with periosteum flagella, facultative anaerobic; catalase, oxidase, ethanol oxidation and V.P. reactions are positive; indole reaction is negative; starch cannot be hydrolyzed, glucose is oxidized Produce acid to coagulate litmus lactic acid. The Genbank accession number of the strain 16S rDNA is AY331578. The direct application of degrading bacteria products can reduce the amount of pesticide residues in crops by more than 90%, solve the problem of excessive pesticide residues in agricultural production, and produce non-toxic and pollution-free green agricultural products.

Description

1. Technical field [0001] The invention discloses a triazophos pesticide residue degrading bacterium and the bacterial agent produced therefrom, belonging to the field of biological high technology, degrading chemical pesticide residues by means of microorganisms, and suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. 2. Background technology [0002] The use of chemical pesticides has greatly promoted the development of modern agriculture, restored a large number of losses caused by diseases and insect pests for agricultural production, and greatly improved the labor efficiency and mechanization degree of agricultural production, playing a role in the modernization of agricultural production. immeasurable effect. But at the same time, pesticides are some toxic and harmful chemicals. After they enter the ecological environment, they not only kill the organisms that are harmful to humans, but also ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20
Inventor 李顺鹏张瑞福蒋建东戴青华崔中利何健
Owner 南京农大环境生物工程有限公司
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