Gold mark detection test paper box for algal toxin and preparation method thereof
A technology for detecting test strips and algal toxins, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of complicated processing and long detection time, and achieve the effects of high sensitivity, fast detection and convenient production.
Inactive Publication Date: 2006-10-18
江苏省苏微微生物研究有限公司
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Problems solved by technology
These methods have disadvantages such as cumbersome sample pretreatment, need for detection equipment, long detection time, high quality and well-trained inspectors, and are not suitable for field operations.
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[0014] 1. Synthesis of antigen MC-KLH: Dissolve 1mg MC-LR in 1mL 0.01M carbonate buffer (pH9.6), dissolve 6mg hemocyanin (KLH) in 6mL 0.01M carbonate buffer , mix the two solutions evenly, then add 1 mL of carbodiimide (EDC) solution (1.5 mg EDC dissolved in 1 mL of 0.01 M carbonate buffer), mix well, and react at room temperature for 2 hours. Transfer the solution into a dialysis belt, dialyze in 0.01M phosphate buffered saline (PBS) dialysate at 4°C, subpackage the dialyzed solution, and store at -20°C.
[0015] 2. Preparation of MC-LR monoclonal antibody
[0016] 1) Immune animals: For 6-8 week old female mice, mix 0.1mg MC-KLH with the same amount of complete Freund's adjuvant for the first immunization, and inject intraperitoneally. After 4 weeks, mix 0.05mg MC-KLH with incomplete Freund's adjuvant and inject intraperitoneally. Thereafter, booster immunization was performed every two weeks, and the method was the same as before. After 12 weeks, 0.05mg MC-KLH intrasplen...
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Abstract
The invention relates to a test paper box for algal toxin gold standard detection and a preparation method thereof, which belong to the technical field of bioengineering products. The test strip of the present invention uses polyvinyl chloride or polyethylene as a backing, and a glass fiber membrane absorbing colloidal gold-anti-MC-LR monoclonal antibody conjugate is pasted on the sample injection area at one end of the test strip; nitrocellulose is pasted on the middle test area Plain film, spray MC-BSA on the film as the detection line, spray goat anti-mouse IgG as the quality control line, and then seal the nitrocellulose film with 1% BSA; paste absorbent paper on the other end of the absorbent area. The test strips are packaged in a box housing correspondingly provided with a sample injection hole and an observation hole. After the test sample is dropped into the sample injection hole, according to the discoloration of the detection line, it is detected whether the algae toxin content in the sample reaches the standard. The invention has the advantages of not needing a large detection instrument, no pretreatment of samples, convenient manufacture of a test paper box, rapid, accurate, obvious and high sensitivity detection.
Description
technical field [0001] The invention relates to a test paper box for algal toxin gold standard detection and a preparation method thereof, which belong to the technical field of bioengineering products. Background technique [0002] Microcystins (MCYST or MC for short) have become one of the pollutants that are widely concerned in environmental water bodies. The Chinese Academy of Sciences "2002 Scientific Development Report" specifically discussed that the greatest harm caused by "water blooms" in fresh water is drinking water. source is threatened. Algal toxins affect human health through the food chain, and the secondary metabolite MC of cyanobacteria "bloom" can damage the liver, has a cancer-promoting effect, and directly threatens human health and survival. For more than 100 years, the human and animal poisoning incidents caused by algae toxins have attracted worldwide attention, and there have been reports in Canada, the United Kingdom, the United States, Australia, ...
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IPC IPC(8): G01N33/558G01N33/531
Inventor 赵晓联孙蔚榕陆茂林赵春城龚燕蔡建荣张东升蔡正森
Owner 江苏省苏微微生物研究有限公司