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Breeding method of Wedelia trilobata

A technology for wedwigs and weeds, which is applied in the field of rapid propagation of the in vitro culture of African wedges by using tissue culture technology, can solve problems such as unreported and unreported tissue culture methods, and ensure the quality of adventitious roots, good growth, and reproduction. Efficient effect

Inactive Publication Date: 2006-11-22
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tissue culture method of asexually propagated seedlings of Wedelia africa has not been reported in China, nor has it been reported in the world

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Disinfection of explants: take the terminal buds or lateral buds of Wedelia gerbera, soak them in 70% ethanol for 30 seconds, sterilize them in 0.1% mercuric chloride for 6 minutes, and rinse them with sterile water for 5 times;

[0019] (2) Acquisition of sterile bacteria: Inoculate the sterilized Wedelia explants on MS medium and cultivate them for 15 days to obtain sterile seedlings. The culture conditions are: temperature 25±2°C, light intensity 1500Lux, light time 12h d -1 (meaning "hour / day", the same below);

[0020] (3) Differentiation of buds: one month later, they were inoculated on the MS medium supplemented with 0.5 mg / L 6-BA, cultivated for 15 days, and the differentiation rate of adventitious buds was 87.5%, and each explant capable of differentiation had 1~ 2 adventitious buds were cultivated for 35 days, the differentiation rate of adventitious buds was 100%, and each explant capable of differentiation had 2 adventitious buds.

[0021] (4) Formatio...

Embodiment 2

[0024] Other operations are the same as in Example 1, except that in the step (1), the terminal buds or lateral buds of Wedelia gerbera are taken and soaked in 65% ethanol for 20 seconds, sterilized in 0.1% mercuric chloride for 4 minutes, and rinsed with sterile water for 6 minutes. time; in the step (2), the culture time was 20 days; in the step (3), the medium formula was MS+0.5mg / L 6-BA+0.1mg / LNAA, and the culture time was 15 days, and the adventitious bud differentiation rate was 100%, each explant has 2 to 3 differentiated buds, and when culturing for 35 days, each explant has an average of 3 differentiated buds; in step (4), the medium is changed to 1 / 2MS+1.0mg / L NAA, when cultivating for 5 days, the rooting rate is also 100%, and the induced roots of each explant are 8 to 9, and the induced roots grow out radially, and the growth condition is good. After cultivating for 10 days, the induction root of each explant Roots increased to 13-14, after 20 days of cultivation, ...

Embodiment 3

[0026] Other operations are the same as in Example 1, except that in step (1), the terminal buds or side buds of Wedelia gerbera are taken and soaked in 75% ethanol for 60 seconds, sterilized in 0.1% mercuric chloride for 12 minutes, and rinsed with sterile water for 3 minutes. time; in the step (2), the culture time was 30 days; in the step (3), the medium formula was MS+0.5mg / L 6-BA+0.2mg / L NAA, and when the culture time was 15 days, the adventitious bud differentiation rate 75%, each explant capable of differentiation has 2 to 3 differentiated buds, and when the culture time is 35 days, the adventitious bud differentiation rate is 100%, and each explant has 3 to 5 differentiated buds; step (4 ), the medium was changed to 1 / 2MS+0.5mg / L NAA, and when cultured for 5 days, the induced roots grew radially, and the rooting rate was also 100%. The induced roots of each explant were 7 to 8, and 10 Three days later, the induced roots of each explant increased to 9-10, and after 20 d...

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Abstract

The propagation method of Wedelia africanus comprises five steps: (1) explant disinfection: adopt 65-75% ethanol and 0.1% mercuric chloride to sterilize; (2) obtain aseptic seedling: adopt MS culture medium, cultivate 15~30 days , temperature 25±2℃, light intensity 1500Lux, light time 12h・d -1 (3) Inducing clustered shoots to take place: the medium is MS+0.5-5mg / L 6-BA+0-0.5mg / LNAA, cultivated for 15-35 days, and the conditions are the same as above; (4) Inducing rooting: the medium is 1 / 2MS+0-5mg / L IBA or NAA, cultivated for 5-20 days, the conditions are the same as above; (5) Transplanting of regenerated plants: transplanting of regenerated plants after hardening. The method can realize the in vitro rapid propagation of the African wedel, and is easy to operate.

Description

(1) Technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for rapid propagation of wedelia africana in vitro culture by using tissue culture technology. (2) Background technology [0002] Plant tissue culture refers to the process of using any organ, tissue or cell of a plant to carry out sterile culture growth and development under artificial control conditions. The technology takes less materials, the cost of cultivating plant materials is low, the growth cycle is short, and the management is convenient. Utilizing the rapid propagation technology of plant tissue culture, a large number of plants that maintain the biological characteristics and genetic traits of the female parent can be reproduced in a short period of time. It is understood that there are nearly a thousand species of fast-growing plants in my country. There are several kinds of basic medium commonly used, such as MS, B5, White, N6, etc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 谭武贤刘吉升陈刚李玲
Owner SOUTH CHINA NORMAL UNIVERSITY
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