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Rapid propagation method of torenia flava

A technology for butterfly grass and yellow flower, which is applied to the field of in vitro rapid propagation of butterfly grass by tissue culture technology, can solve many problems such as not many, and achieves the effects of simple operation, guaranteeing the quality of adventitious roots, and high reproduction efficiency.

Inactive Publication Date: 2012-08-15
ZHAOQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the domestic researches on Phalaenopsis chrysalis as experimental materials are still in the initial stage, and only preliminary researches have been carried out on in vitro culture conditions.
Although there are reports on the plant regeneration of Phalaenopsis chrysalis, there are not many

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Disinfection of explants: take the terminal buds or lateral buds of Pantera japonica, soak them in 70% ethanol for 30 seconds, sterilize them in 0.1% mercury liter for 6 minutes, and rinse them with sterile water for 5 times;

[0024] (2) Acquisition of sterile seedlings: Inoculate the explants of the sterilized Phalaenopsis chrysalis in MS medium and cultivate them for 15 days to obtain sterile seedlings. The culture conditions are: temperature 25±2°C, light intensity 1500Lux, light time 12h ·d-1 (indicates "hour / day", the same below).

[0025] (3) Bud differentiation: one month later, inoculated on the medium of MS+1.0mg / L 6-BA+1.5mg / L NAA, and cultured for 14 days, the differentiation rate of adventitious buds was 97.62%. The implant has 12 adventitious buds, and after 21 days of culture, the differentiation rate of adventitious buds is 100%, and each explant that can differentiate has more than 30 adventitious buds.

[0026] (4) Root formation: The formed seedl...

Embodiment 2

[0029] Other operations are the same as in Example 1, the difference is: in step (1), the top buds or side buds of Pantera japonica are taken and soaked in 75% ethanol for 60 seconds, sterilized in 0.1% mercury liter for 12 minutes, and then rinsed with sterile water 3 times; in step (2), the culture time was 30 days; in step (3), the medium formula was 2.0 mg / L 6-BA MS medium, cultured for 14 days, and the adventitious bud differentiation rate was 94.64 %, each explant has 8 adventitious buds, cultivated for 21 days, the adventitious bud differentiation rate is 100%, and each explant that can be differentiated has more than 20 adventitious buds; in the step ((4), the medium is changed to On 1 / 3MS medium with 0.1mg / L NAA, when cultured for 7 days, the rooting rate was 93.54%, and each explant had 6-7 induced roots. After 14 days of culture, the rooting rate could reach 100%. The induced root of each explant is greater than 11, and it is radial. The growth condition of the seed...

Embodiment 3

[0031] Other operations are the same as in Example 1, except that in step (1), the top buds or side buds of Paleopsis chrysalis are taken and soaked in 65% ethanol for 20 seconds, sterilized in 0.1% mercury liter for 4 minutes, and then rinsed with sterile water 6 times; in step (2), the culture time was 20 days; in step (3), the medium formula was 0.01mg / L 4-PU MS medium, cultured for 14 days, the adventitious bud differentiation rate was 86.77 %, each explant has 8 adventitious buds, cultivated for 21 days, the adventitious bud differentiation rate is 100%, and each explant capable of differentiation has 34 adventitious buds; in the step ((4), the medium is changed to 1 / 3MS, when cultured for 7 days, the rooting rate was 90%, and each explant had 5 induced roots; after 14 days of culture, the rooting rate could reach 100%, and each explant had 6~ 7 roots, and the growth condition is good, showing a radial shape. The growth condition of the seedlings is good.

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Abstract

The invention relates to a rapid propagation method of torenia flava, comprising the following steps of: (1) disinfecting an explant with 65-75% ethanol and 0.1% mercury; (2) obtaining sterile seedlings: cultivating for 15-30 days with an adjusted MS culture medium under the condition that the temperature is 25+ / -2 DEG C, the light illuminating strength is 1500 Lux, and the light illuminating time is 12 hours per day; (3) inducing the generation of cluster buds: using the adjusted MS culture medium, cultivating for 14-21 days, wherein the condition is the same as above; (4) inducing to root: cultivating with the adjusted MS culture medium for 7-14 days on the same condition as the step (3); and (5) transplanting regeneration plants: transplanting after acclimatizing the regeneration plants. The method can obviously improve asexual reproduction coefficient of the torenia flava, and realize the scale micro-propagation of the torenia flava; the method has the advantages of high propagation speed and is convenient to operate; and the method is an effective path for obtaining a large number of excellent seedlings.

Description

technical field [0001] The invention relates to a tissue culture method, in particular to a method for in vitro rapid propagation of Paleopsis chrysalis by adopting tissue culture technology. Background technique [0002] Plant tissue culture refers to the process of using any organ, tissue or cell of a plant to carry out sterile culture growth and development under artificial control conditions. The technology takes less materials, the cost of cultivating plant materials is low, the growth cycle is short, and the management is convenient. Utilizing the rapid propagation technology of plant tissue culture, a large number of plants that maintain the biological characteristics and genetic traits of the female parent can be reproduced in a short period of time. It is understood that there are nearly a thousand species of fast-growing plants in my country. There are several kinds of basic medium commonly used, such as MS, B5, White, N6, etc. In tissue culture, under the actio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈刚王瑛华陈雄伟林媛
Owner ZHAOQING UNIV
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