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Breeding method for pachystachys lutea

A shrimp flower and formula technology, applied in the field of plant tissue culture, can solve the problems of low differentiation rate, low reproduction coefficient, low reproduction coefficient, inconvenient operation, etc., and achieve the effects of ensuring the quality of adventitious roots, high reproduction efficiency and easy operation.

Inactive Publication Date: 2007-10-03
SOUTH CHINA NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

) But there are certain shortcomings in the culture method of three examples of Huangshihua: the differentiation rate of the first example is low, and the reproduction coefficient is not high; the second example only uses MS+3mg / L 6-benzylaminopurine (6-BA for short )+0.1mg / L naphthaleneacetic acid (abbreviated as NAA) to induce differentiation buds; the third example uses a wide variety of hormones, which is inconvenient to operate, and the differentiation rate and reproduction coefficient are not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Disinfection of explants: take yellow shrimp flower terminal buds or lateral buds, soak them in 65% ethanol for 90 seconds, sterilize them in 0.1% mercuric chloride for 12 minutes, and rinse them with sterile water for 6 times;

[0020] (2) Acquisition of sterile seedlings: inoculate the sterilized yellow shrimp flower explants on MS medium and cultivate them for 30 days to obtain sterile seedlings. The culture conditions are: temperature 25±2°C, light intensity 1500Lux, light time 12h·d -1 (meaning "hour / day", the same below);

[0021] (3) Differentiation of buds: one month later, they were inoculated on the MS medium supplemented with 1 mg / L 6-BA, cultivated for 10 days, the differentiation rate of adventitious buds was 100%, and each explant capable of differentiation had 1 to 3 Adventitious buds were cultivated for 25 days, and each explant that could differentiate had 4 adventitious buds.

[0022] (4) The formation of root: the seedling that forms, transfers ...

Embodiment 2

[0025] Other operations are the same as in Example 1, the difference is: in step (1), take yellow shrimp flower terminal buds or lateral buds and place them in 70% ethanol to soak for 60 seconds, sterilize in 0.1% mercury liter for 8 minutes, and then rinse with sterile water 5 times; in step (2), the culture time was 25 days; in step (3), the medium formula was MS+1mg / L 6-BA+0.1mg / L NAA, the culture time was 10 days, and the adventitious bud differentiation rate 100%, each explant has 2~4 differentiated buds, and when culturing for 25 days, each explant has 6 differentiated buds on average; in step (4), the medium is changed to 1 / 2MS+1.0mg / LNAA, when cultivating for 5 days, the rooting rate was 75%; after cultivating for 10 days, the rooting rate was 100%, and the roots radiated out, thicker and shorter, in light yellow color, and after cultivating for 20 days, the induced root growth became longer, and the growth condition of the seedlings was good; In the step (5), the ver...

Embodiment 3

[0027] Other operations are the same as in Example 1, the difference is: in step (1), take yellow shrimp flower terminal buds or side buds, soak them in 75% ethanol for 30 seconds, sterilize them in 0.1% mercuric chloride for 4 minutes, and rinse them with sterile water 3 times; in step (2), the culture time was 20 days; in step (3), the medium formula was MS+1mg / L 6-BA+0.2mg / L NAA, and when the culture time was 10 days, the adventitious bud differentiation rate 100%, each explant capable of differentiation has 3 to 4 differentiated buds, and when the culture time is 25 days, each explant has 5 to 7 differentiated buds; in step (4), the medium is changed to 1 / 2MS+0.5mg / L NAA, when cultured for 5 days, the roots are induced to grow radially, and the rooting rate is 50%. , induce root growth to become longer, and the growth of seedlings is in good condition.

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Abstract

The breeding process of pachystachys lutea includes the following five steps: 1. sterilizing explant with 65-75 % concentration alcohol solution and 0.1 % concentration mercuric chloride solution; 2. culturing abacterial seedling in MS culture medium at 25 deg c temperature, lighting strength of 1500 Lux and lighting time of 12 hr / day for 15-30 days; 3. inducing cespitose bud through culturing in MS+(1-3) mg / L 6-BA+(0-0.3) mg / L NAA culture medium in the same conditions as in the step 2 for 10-25 days; 4.inducing rooting through culturing in 1 / 2MS+(0-1) mg / L 6-IBA or NAA culture medium in the same conditions as in the step 2 for 5-20 days; and 5.transplanting the regenerated plant after hardening off. The said process can realize the fast isolated breeding of pachystachys lutea and has simple operation.

Description

(1) Technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for rapid propagation of yellow shrimp flowers in vitro culture by using tissue culture technology. (2) Background technology [0002] Plant tissue culture refers to the process of using any organ, tissue or cell of a plant to carry out sterile culture growth and development under artificial control conditions. The technology takes less materials, the cost of cultivating plant materials is low, the growth cycle is short, and the management is convenient. Utilizing the rapid propagation technology of plant tissue culture, a large number of plants that maintain the biological characteristics and genetic traits of the female parent can be reproduced in a short period of time. It is understood that there are nearly a thousand species of fast-growing plants in my country. There are several kinds of basic medium commonly used, such as MS, B5, White, N6...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 刘吉升谭武贤陈刚李玲
Owner SOUTH CHINA NORMAL UNIVERSITY
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