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Fast excised African chrysanthemum porpagation method

A kind of gerbera, in vitro technology, applied in the field of plant tissue culture, can solve the problems of no herbaceous plants, achieve good growth, shorten the occurrence time, and ensure the quality of adventitious roots

Inactive Publication Date: 2004-01-07
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Another report pointed out that before the in vitro culture of woody plant buds, low temperature pretreatment for a period of time is beneficial to in vitro culture growth (Huang Xuelin, Li Xiaoju edited. Morphological establishment and regulation of in vitro culture of higher plant tissues, Beijing : Science Press, 1995, 78), but there is no identical report on herbaceous plant aspect at present

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 42 receptacles of the Qingxin orange-red variety Gerbera chrysanthemum as explants, first place the flower buds in an environment of 9±2°C for 5 days, then take out the receptacles according to the conventional method for surface disinfection and inoculation culture, the medium is 1 / 2MS, containing 0.5mg / L indole acetic acid and 10mg / L 6-benzyl adenine, found that 18 explants had germinated after 30 days of culture, and the adventitious bud induction rate reached 41.9%. 3.3 buds. The adventitious buds were cut and cultivated into seedlings, and healthy seedlings were obtained, and the growth of adventitious roots was better. And the control group that adopts conventional method to cultivate (without low temperature treatment), the incidence rate of its adventitious buds is only 22.2%, its adventitious roots take place less, grow slowly, obviously worse than the method of the present invention.

Embodiment 2

[0019] Other operations and the control group were the same as in Example 1. There were 56 Qingxin Orange infrared implants, and the time for low-temperature treatment of flower buds was 3 days. After 30 days of cultivation, 24 explants germinated, and the bud induction rate reached 42.9%. There were 6 buds formed on the implant, and after culture and rooting, the growth of adventitious roots was better, and the induction rate of adventitious buds and the growth of adventitious roots were significantly better than those of the control group.

Embodiment 3

[0021] Other operations and the control group are the same as in Example 1. Gerbera adopts the Qingxin Dahong variety, the low-temperature treatment time is 1 day, and the incidence of adventitious buds reaches 14.3% after 30 days of cultivation, and the average number of flower buds formed by each receptacle explant is 2. Cultivate and take root, the growth condition of its adventitious root is normal. And the adventitious bud induction rate of the control group is only 5%, and the flower bud number that each receptacle explant forms on average is 1, and the growth difficulty of adventitious root is obviously worse than the method of the present invention.

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Abstract

The fast excised propagation of African chrysanthemum includes three improved stpes: the disinfection of explant, induced germination of adventitious bud and culture to root. In the disinfection of explant, bud is first treated of low temperature before the surface disinfection of receptacle; in induced germination of adventitious bud, culture medium with phenyl carbamide as cytomin is used; and in culture to root, test tube seedling base part is soaked with auxin solution before culture in solid culture medium containing no hormone. The said method can raise the asexual propagation coefficient of African chrysanthemum through the simple operate process.

Description

(1) Technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for rapid propagation of gerbera in vitro culture by adopting tissue culture technology. (2) Background technology [0002] Plant tissue culture refers to the process of using any organ, tissue or cell of a plant to carry out sterile culture growth and development under artificial control conditions. The technology takes less materials, the cost of cultivating plant materials is low, the growth cycle is short, and the management is convenient. Utilizing the rapid propagation technology of plant tissue culture, a large number of plants that maintain the biological characteristics and genetic traits of the female parent can be reproduced in a short period of time. It is understood that there are nearly a thousand species of fast-growing plants in my country. There are several kinds of basic medium commonly used, such as MS, B5, White, N6, etc. In tissue culture, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 黄群声李玲张铭光
Owner SOUTH CHINA NORMAL UNIVERSITY
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