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One section human type mycoplasmas DNA sequence (1) specificity and its application

A technology of Mycoplasma hominis and DNA sequence, which is applied in the field of biomedicine to achieve the effect of low cost and easy operation

Inactive Publication Date: 2006-11-22
昆明云大生化科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Mycoplasma hominis is a pathogen that frequently infects human genitals and can cause many genitourinary diseases. Accurate diagnosis is the basis for immediate and reliable treatment. At present, it can only be diagnosed according to clinical indications

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  • One section human type mycoplasmas DNA sequence (1) specificity and its application
  • One section human type mycoplasmas DNA sequence (1) specificity and its application
  • One section human type mycoplasmas DNA sequence (1) specificity and its application

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Embodiment Construction

[0019] Firstly, the nucleotide sequence of Mycoplasma hominis was searched in the gene database with Mycoplasma hominis as the keyword. A total of several closely related nucleotide sequences were obtained, and these nucleotide sequences were compared with the nucleotide sequences (nr) in the gene database using the Blast online software on the NCBI network to exclude non-specific nucleotide sequences, A 1 Kb specific nucleotide sequence was obtained. More than 20 pairs of PCR primers were designed based on this sequence, DNA was extracted from clinical specimens, PCR amplification was performed, and a pair of sequences and primers suitable for PCR amplification were screened out. The products obtained by PCR amplification were consistent with the expected size (see figure 1 , the serial numbers in the figure are: 1. Standard molecular weight DNA, the molecular weights from top to bottom are: 622, 527, 404, 309, 242 / 238, 217, 201, 190, 180, 160+160, 147+147, 123 ; 2.4.5 Clini...

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Abstract

The invention relates to a specific DNA sequence (1) of Mycoplasma hominis and an application thereof, belonging to the technical field of biomedicine. The present invention determines the specificity and PCR amplification primer of a segment of Mycoplasma hominis DNA sequence (1) by searching and analyzing the data of the gene database, and the sequence length is 196bp. The sequence was obtained by PCR amplification and cloning of specimens infected with Mycoplasma hominis in the human reproductive tract, and the sequence was confirmed to be consistent with the expected sequence. The results of bioinformatics analysis and gene chip hybridization analysis confirmed the specificity of the sequence to Mycoplasma hominis. The present invention is used for the diagnosis of Mycoplasma hominis as a probe for gene chip diagnosis and a PCR amplification detection sequence. The method used by the clinical detection technology established by the present invention has the advantages of rapidity, accuracy, simple operation, low cost and the like.

Description

Technical field: [0001] The invention relates to a specific DNA sequence (1) of Mycoplasma hominis and its application, belonging to the technical field of biomedicine. Background technique: [0002] Mycoplasma hominis is a pathogen that frequently infects the human genitals and can cause many genitourinary diseases. Accurate diagnosis is the basis for immediate and reliable treatment. At present, it can only be diagnosed according to clinical indications. PCR and gene chip diagnosis is a fast and accurate detection method. Diagnostic methods of this type rely on the availability of specific nucleic acid sequences. Invention content: [0003] The invention is to determine a section of mycoplasma hominis specific nucleotide sequence and its PCR primer through bioinformatics and molecular biology techniques. The sequence and corresponding PCR primers can be used to establish a gene chip and PCR method for diagnosis of mycoplasma hominis. [0004] The technical scheme of t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C07H21/04
Inventor 谭德勇朱宝生
Owner 昆明云大生化科技有限责任公司