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An orthogonal light path type fluorescent detection device for microchip analysis

A fluorescence detection and micro-analysis technology, which is applied in the direction of measuring devices, analysis materials, fluorescence/phosphorescence, etc., can solve the problems of complex optical system structure, difficulty in miniaturization, high price, etc., achieve high detection sensitivity, reduce light scattering, Inexpensive effect

Inactive Publication Date: 2006-11-22
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The advantage of the confocal fluorescence detector is that the detection sensitivity is high, but this type of optical system has a complex structure, is expensive, and is not easy to miniaturize and integrate.

Method used

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  • An orthogonal light path type fluorescent detection device for microchip analysis
  • An orthogonal light path type fluorescent detection device for microchip analysis
  • An orthogonal light path type fluorescent detection device for microchip analysis

Examples

Experimental program
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Embodiment 1

[0024] Refer to attached figure 1 , The microchip is composed of upper 1 and lower 2 glass sheets, and the thickness of the glass sheets is 1.7 mm. A channel is processed on the upper sheet 1 by photomask and wet etching technology, and the width of the microchannel 6 is 60 microns, and the depth is 20 microns. The classic high-temperature bonding method is used to realize the permanent sealing of the upper 1 and lower 2 pieces. A microfluidic chip with a length of 60 mm and a width of 15 mm was obtained. In the present embodiment, the excitation light source 7 is a semiconductor pump laser of 473nm; the excitation light 5 emitted by the laser is focused by the condenser objective lens 4, and enters the chip microchannel 6 from a direction perpendicular to the plane of the lower chip 2 of the chip; 11 emitted, the fluorescence 8 emitted from the chip side wall 3 is collected by the collection objective lens 9 positioned behind the detection window, and the setting direction ...

Embodiment 2

[0027] Refer to attached image 3 After the excitation light 5 emitted by the light source 7 is converged by the focusing objective lens 4, it is incident from the side wall 3 of the chip in a direction perpendicular to the microchannel 6 of the chip; the fluorescent light 8 emitted from the direction perpendicular to the lower chip 2 of the chip is selected, and collected The objective lens 9 collects the stray light, and the stray light is filtered out by the optical filter, and finally the fluorescent signal is detected by the photodetector 10 .

[0028] Figure 4 It is the channel configuration diagram of the micro-analysis chip according to the preferred embodiment 1 of the present invention. The separation channel part of the microchannel 6 on the chip is designed with a bend, near the edge of the side wall 3 of the chip at the end detection area 12, in order to shorten the distance of fluorescence from the light spot to the detection window and improve the collection e...

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Abstract

An orthogonal optical path fluorescence detection device for micro-analysis chips, the device is composed of a light source, an optical system, and a detection system, and is characterized in that the optical system adopts an orthogonal detection optical path, and the excitation light emitted by the light source The incident side wall of the analysis chip is analyzed, and the detection system detects the fluorescence emitted through the side wall of the chip. The invention has the advantages of simple optical system of the device, easy processing, low manufacturing cost, high detection sensitivity, easy integration and miniaturization of the system.

Description

technical field [0001] The invention relates to microfluidic chip analysis, in particular to an orthogonal optical path fluorescence detection device for a microanalysis chip. Background technique [0002] The field involved in this patent is the research on the detection system on the micro-analysis chip. The detection system is an important part of an analysis system. Due to the small size of the micro-analysis chip, the injection volume is only picoliters or nanoliters, and the reaction channel at the detection site is generally tens of microns wide, so the requirements for its detection methods and devices have its own particularity. [0003] At present, one of the main detection methods used in micro-analysis chips is the fluorescence method. Among them, the laser-induced fluorescence (Laser Induced Fluorescence, LIF) detection method using a laser as the excitation light source has the characteristics of high sensitivity and easy detection on the chip. and become the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N21/00H01L21/66
Inventor 方群富景林黄艳贞
Owner ZHEJIANG UNIV
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