Functional nano-rare earth fluorescent micro particle and its preparation and application

A technology of nano-rare earth and fluorescent particles, which is applied in the field of nano-rare-earth fluorescent materials, can solve the problems of unsuitable nano-fluorescent particles, low measurement sensitivity, weak fluorescence, etc., and achieve the effects of improving measurement sensitivity, solving fluorescence bleaching, and enhancing photostability

Inactive Publication Date: 2007-02-07
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantage of using rare earth fluorescent compounds directly as markers is that the fluorescence quantum yields of such compounds are generally small and the fluorescence is weak. However, it is impossible to greatly improve the sensitivity of time-resolved fluorescent biodetection technology only by improving the fluorescence quantum yield of rare earth fluorescent markers.
Due to the high concentration of fluorescent compounds contained in the fluorescent nanoparticles, theoretically using fluorescent nanoparticles as markers can solve the problem of low detection sensitivity when weaker fluorescent compounds are directly used as markers. Due to the problem of extinction, it is not suitable for the preparation of fluorescent nanoparticles containing higher concentrations of fluorescent compounds

Method used

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  • Functional nano-rare earth fluorescent micro particle and its preparation and application
  • Functional nano-rare earth fluorescent micro particle and its preparation and application
  • Functional nano-rare earth fluorescent micro particle and its preparation and application

Examples

Experimental program
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Effect test

Embodiment 1

[0048] Contains 4'-(2-thienyl)-2,2':6',2"-biteropyridine-6,6"-dimethylaminetetraacetic acid europium (TTTA-Eu for short) 3+ ) Preparation of nano fluorescent particles:

[0049] (1) TTTA-Eu 3+ Preparation of complexes

[0050] 411 mg of TTTA·H 2 O was dissolved in 6ml of water, with solid NaHCO 3 Adjust the pH to 6.5. Then 116 mg of Eu 2 o 3 After heating and dissolving with dilute hydrochloric acid, add it to TTTA aqueous solution, and use solid NaHCO 3 Adjust the pH to 6.5, stir at room temperature for 2 hours, remove the precipitate by filtration, add 300ml of acetone, stir at room temperature for 1 hour, collect the precipitate by filtration, dry in vacuo, wash the dry solid with dry methanol, evaporate the filtrate to dryness under reduced pressure, and dry in vacuo. 470mg of TTTA-Eu can be obtained 3+ Complex (yield 67.7%).

[0051] (2) Contains europium complex TTTA-Eu 3+ Preparation of fluorescent nanoparticles

[0052] The europium fluorescent complex TTTA...

Embodiment 2

[0062] Use TTTA-Eu 3+ Determination of alpha-fetoprotein (AFP) in human serum by time-resolved fluorescent immunoassay with complex LFNP as marker

[0063] (1) Use nano-rare earth fluorescent particles to label streptavidin (SA)

[0064] Disperse the nanospheres to 2mol / L Na 2 CO 3 In the aqueous solution, after 20 minutes of ultrasonic activation, add the acetonitrile solution dissolved in CNBr, react at room temperature for 2-10 minutes, centrifuge to collect the precipitate, and use ice water and 0.1mol / L NaHCO for the precipitate 3 (pH=8.5) buffer solution was washed twice, and then the activated nanoparticles were added to the aqueous solution containing bovine serum albumin (abbreviated as BSA), and reacted overnight, with 0.1mol / L NaHCO 3(pH=8.5) After washing with buffer solution, add SA and glutaraldehyde to react for 24 hours, then add NaBH 4 Reduction, react at room temperature for 30 minutes, wash several times and suspend in solution containing 0.2% BSA-0.1% N...

Embodiment 3

[0072] Contains N, N, N 1 , N 1 -[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-(2"-phenyl)pyridine]terbium tetraacetate (referred to as BPTA-Tb 3+ ) preparation of fluorescent nanoparticles:

[0073] (1) BPTA-Tb 3+ Preparation of complexes

[0074] Dissolve 192 mg of BPTA (see Document 7 for the synthetic method: Yuan J, Wang G, Majima K, Matsumoto K.nal.Chem.2001, 73, 1869-1876) in 4 ml of water, and use NaHCO 3 Adjust the pH to 6.5. Then 120mg of TbCl 3 The solution dissolved in 2ml of water was added to the BPTA aqueous solution, with NaHCO 3 Adjust the pH to 6.5, stir at room temperature for 2 hours, remove the precipitate by filtration, add 300ml of acetone to the filtrate, stir at room temperature for 1 hour, collect the precipitate by filtration, dry in vacuo, wash the dry solid with dry methanol, evaporate the filtrate to dryness in vacuo After drying, 198mg of BPTA-Tb can be obtained 3+ Complex (72.9% yield).

[0075] (2) Contains BPTA-Tb 3+ Preparation of Fluores...

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Abstract

A functional lanthanide fluorescence nanoparticle (LFNP) is prepared from the strong-fluorescent RE matches as luminous center through chemically wrapping by silica gel. Its advantages are long fluorescence lift, big stoke shift, narrow emitting peak and strong specific signal. It can be used as the fluorescent marker for biological detection.

Description

technical field [0001] The invention relates to a nano-rare-earth fluorescent material, in particular to a functional nano-rare-earth fluorescent particle and its preparation and application. Background technique [0002] Bioanalytical chemistry at the nanoscale is one of the most important development directions of nanobiotechnology. It is the research frontier and development direction in the field of international bioanalysis science, and it is a research hotspot in various countries. Functional nano-fluorescent materials are a new growth point in the research of nano-materials in recent years, and their application focus is currently concentrated in the field of biotechnology, such as single-molecule in situ detection of biological macromolecules, fluorescence microscopy detection, immunohistochemistry, cytochemistry, etc. . The reported technology and its existing problems include the following aspects: [0003] (1) Nanoscale ZnS-CdSe fluorescent particle (also known ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/77
Inventor 袁景利谭明乾叶志强王桂兰
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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