Method of inducing an anti-tumor response against a lung metastasis in a melanoma patient
A melanoma and anti-tumor technology, applied in anti-tumor drugs, pharmaceutical formulations, cancer antigen components, etc., can solve problems such as high dose of BCG, excessive dose of leukemia cells, and unsuccessful conventional treatment
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Embodiment 1
[0072] Sixteen patients with melanoma with lung metastases were treated according to the present invention. Lung metastases were detectable in all treated patients. For the purposes of the present invention, the term "detectable" pulmonary metastases refers to x-ray visible metastases. Melanoma cells were isolated from lymph nodes and lung metastases and prepared as described above. Cells were obtained by enzymatic dissociation of metastatic diseased masses (with collagenase and DNase). Cells were bound to DNP as described herein. Some patients were sensitized to DNP by topical application of 5% dinitrochlorobenzene to the upper arm. Low-dose cyclophosphamide (CY) preceded the first vaccine. On day 0, the patient was given an intravenous injection of 300Mg / M 2 of cyclophosphamide. Three days later, an intradermal injection containing 2.5 x 10 6 to 25×10 6 Vaccine of autologous cryopreserved irradiated (2500R) tumor cells. Most patients were treated weekly for a total ...
Embodiment 2
[0075] Tumor masses used to prepare vaccine compositions of the invention can be obtained from lymph node, lung or subcutaneous metastatic masses and prepared as described above. Briefly, cells can be extracted by enzymatic dissociation with collagenase and DNase and by mechanical dissociation. Cell membranes can be isolated as described in this manual, frozen in a controlled rate freezer, and stored in liquid nitrogen for later use. On the day patients were treated, membranes were thawed, washed and resuspended in Hanks' balanced salt solution without phenol red. Modification with DNP can be performed by the method of Miller and Claman (1976). The method involves incubating melanoma cells with dinitrofluorobenzene (DNFB) for 30 minutes under sterile conditions followed by washing with sterile saline.
[0076] Patients with small and multiple lung metastases melanoma and patients with lung metastases can be treated as follows. The vaccine composition may contain a minimum o...
Embodiment 3
[0083] The four patients who responded to the vaccine of the invention according to Example 1 continued to be monitored to evaluate the potency of the vaccine. Three patients (patients 2, 3 and 4 in Example 1) received a total of 6 vaccinations, once a week, and a booster vaccine at 6 and 12 months from the initial vaccination. One patient (Patient 1) received weekly vaccines for twelve weeks without booster vaccines. Two patients had complete resolution and two patients had partial resolution. Patient 1 (in Example 1) had partial regression for 12 months. Patient 2 (in Example 1) had partial regression for 8 months. Patient 3 (of Example 1) had complete remission for 29 months and the fourth patient (of Example 1) had complete remission for more than 27 months. The term "complete regression" refers to complete disappearance of all detectable tumors. The term "partial regression" generally refers to at least a 50% reduction in tumor diameter. In any case, in the context o...
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