Preparation method of glutaryl-7-aminocefaphytanic acid acyltransferase

Abstract

The method for preparing glutaryl-7-aminocephaphylanic acid acylase is characterized by that it utilizes colibacillus gene engineering bacterium to make shake-flask strain culture in culture medium, fermentation strain culture and fermentation culture, then utilizes centrifuge to collect thallus, soakes to extract enzyme liquor, makes the enzyme liquor undergo the processes of plate-frame filtration, ultrafiltration concentration, purification and fixation so as to obtain the invented product GL-7-ACA ACY.

Description

Technical field:

[0001] The invention relates to a preparation method of glutaryl-7-aminocephalosporanic acid acylase used for the key intermediate 7-aminocephalosporanic acid in the synthesis of semi-synthetic cephalosporins. Background technique:

[0002] Cephalosporins are antibiotics with important efficacy, while semi-synthetic cephalosporins have stronger antibacterial ability or can improve pharmacokinetic properties. And the key intermediate of semi-synthetic cephalosporin is 7-aminocephalosporanic acid (7-ACA), the method of traditional production in the past mainly is by chemical method, has four major steps, and process is complicated, and used reagent is expensive, and pollution is serious, And the yield is low and the quality is poor. The biotransformation method is a better method. Studies have shown that cephalosporin C can be oxidatively deaminated by D-amino acid oxidase, and further decarboxylated to generate GL-7-ACA, while the 7-side chain of GL-7-ACA c...