Fast separation device of pine material nematode and its detection method

Abstract

A high-speed separator for detecting pine nematode is composed of a pumping filter barrel, a sleeve tube and a vacuum pump communicated with the tube on the wall of pumping filter barrel. Its detecting method includes such steps as embedding said pumping filter barrel and sleeve tube in pine wood to be detected, filling water in sleeve tube, pumping water in the pumping filter barrel, and detecting pine nematode by microscope. Its advantage is high speed (20-30 min).

Description

(1) Technical field [0001] The invention relates to a rapid separation device for the pathogenic nematode of pine wood nematode and a detection method thereof. (2) Background technology [0002] The pine wood nematode (Bursaphelenchus xylophilus) causes pine wilt disease, known as "smokeless forest fires" or "pine cancer". Because pine xylophilus reproduces quickly and spreads through vector insects such as longhorns, it quickly spreads and becomes a disaster, causing large areas of pine trees to die quickly. Therefore, prohibiting the movement of wood in the diseased area is one of the main measures to prevent the spread of the disease. In addition, many wooden packaging boxes for imported and exported goods are made of pine wood, which is also the main cause of the disease. In order to prevent the further spread of the disease, checkpoints have been set up on major traffic arteries across the country to intercept diseased pine trees; each port is also closely inspecting ...

AI Technical Summary

Problems solved by technology

Jian Yongxun et al. (Chinese Forest Diseases and Pests, 2001, No. 4, 5-6, 23) reported "Research on Sampling Methods for Quarantine Inspection and Inspection of Pine Wood Nematode". The method cannot get rid of the "Bellman funnel", so it still takes more than 20 hours to produce results

At various checkpoints, cargo owners and car drivers are extremely reluctant to spend more than a day at the checkpoint, which means huge economic losses

The loss caused by port quarantine due to waiting for the result is even greater

Therefore, the rapid detection of pine wood nematode is an urgent task to be solved. Therefore, during the "Eighth Five-Year Plan" and "Ninth Five-Year Plan", my country's forestry department once set up a project to tackle key problems. Changed to judge the presence of nematodes (Wang Yuyan et al. "Test of two indicators for pine wood nematode disease wood", oral communication materials of the Fourth National Symposium on Plant Nematology), but it was not accepted because the sensitivity did not meet the requirements. use

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