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Method of quickly improving paddy rice quality

A rice, rapid technology, applied in the fields of plant genetic improvement, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problem of not tracking the size of imported resistance gene fragments, and achieve improved rice quality, accurate rice quality, The effect of shortening the breeding process

Inactive Publication Date: 2004-02-18
HUAZHONG AGRI UNIV
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Problems solved by technology

However, they neither tracked the size of the introduced resistance gene segment, nor did they perform selection on the genetic background outside the resistance gene segment, so that the improved rice varieties and the improved varieties have great changes in agronomic traits, and it is difficult to Enables the direct application of improved new rice varieties to production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Genetic improvement of eating taste and cooking quality of hybrid rice maintainer line "Zhenshan 97":

[0033] (1) The line Minghui 63 containing the wx gene with good taste and cooking quality, medium amylose content, and soft gel consistency was used as the donor parent, and the most widely used maintainer line "Zhenshan 97B" in China was used as the recurrent parent. are crossed, resulting in F 1 Then backcross with the reincarnated parent to get BC 1 f 1 ;

[0034] (2) to BC 1 f 1 Perform PCR analysis on individual plants, select those with wx gene for AFLP analysis, select the individual plant with the most genetic background of the recurrent parent and backcross with the recurrent parent to obtain BC 2 f 1 ;

[0035] (3) to BC 2 f 1 Perform PCR analysis on individual plants, select individual plants containing the wx gene and exchange with the molecular marker C952 on one side of the gene, and then backcross with the recurrent parent to obtain BC 3 f 1 ...

Embodiment 2

[0039] Identification results of cooking and eating quality of seeds (rice) of different rice varieties such as "Minghui 63", "Zhenshan 97" and "Improved Zhenshan 97":

[0040] The applicant used the rice seeds harvested in Wuhan, Hubei, China in 2000 and the rice seeds harvested in Hainan, China in 2001 to analyze the rice quality traits of the "Improved Zhenshan 97B" line, and the results are listed in Table 1. Compared with "Zhenshan 97B", in terms of cooking and eating quality, the test results of the rice seeds harvested in Wuhan, Hubei: the amylose content decreased from 27.8% to 16.08%, and the gel consistency increased from 29.5mm to 53.3mm, which was the same as " The amylose content of Minghui 63” is 16.9%, and the gel consistency is 53.8mm, which is very consistent; the test results of rice seeds harvested in Hainan show that the amylose content decreased from 27.9% to 15.9% on average, and the gel consistency increased from 28.1mm on average It reaches 54.28mm, whi...

Embodiment 3

[0043] Comparative identification of the agronomic traits of the "improved Zhenshan 97" variety cultivated in the present invention and the original "Zhenshan 97":

[0044] The comparison test results in the summer of 2000 in Wuhan (Table 2) show that the main agronomic traits of the "improved Zhenshan 97B" and the original "Zhenshan 97B" are basically the same as those of the original "Zhenshan 97B". In the natural growing area of ​​Hainan in 2001, the applicant compared the agronomic characters of "Improved Zhenshan 97B" and "Zhenshan 97" and obtained the same result. Although there were slight differences in some agronomic traits, the statistical analysis showed that the differences were not significant. It shows that the effect of using molecular marker-assisted selection to improve rice quality is obvious, but the agronomic traits are not significantly different from the original control varieties.

[0045] Table 2 Comparison of agronomic traits between "improved Zhensha...

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Abstract

A method for quickly improving the variety of paddy rice to increase the content of moderate straight-chain starch and maltha in paddy rice by molecular marker aided selection includes hybridizing, backcross, quality identification, and molecular marker detection. Its advantages are short period (5 generations) and no change of other characteristics.

Description

Technical field: [0001] The invention belongs to the technical field of rice quality breeding. It relates to the technical field of conventional hybrid breeding and molecular marker-assisted selection breeding of rice. Background technique: [0002] The main goal of rice improvement is high yield, high quality, and multi-resistance. However, in the past quite a long period of time, in order to solve the food shortage in my country, the yield has always been the main goal in the breeding process. This kind of high-yielding breeding with yield as the main goal has made some of the main varieties popularized in my country, especially hybrid rice varieties, of poor quality (Mo Huidong, Improvement of Rice Quality in my country, "Chinese Agricultural Science", 1993, 26(4): 8-14; Zhou Kaida, Li Hongwei, Cheng Yu, The only way to high-quality hybrid rice, "Hybrid Rice", 1994, (3-4): 42-45; Yang Zhenyu, Progress in japonica hybrid rice breeding, " Hybrid Rice", 1994(3-4): 46-49). ...

Claims

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Application Information

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IPC IPC(8): A01H1/02C12Q1/68
Inventor 何予卿周鹏辉谈移芳徐才国张启发
Owner HUAZHONG AGRI UNIV
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