KASP molecular marker co-segregated with powdery mildew resistant gene Pm58 and application of KASP molecular marker

A powdery mildew resistance gene and molecular marker technology, applied in the field of crop breeding, can solve the problems such as the difficulty of using excellent disease-resistant alleles, and achieve the effects of solving linkage burden, stable amplification, and convenient detection

Pending Publication Date: 2022-03-25
HENAN UNIVERSITY
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention proposes a kind of powdery mildew resistance gene Pm58 The co-segregated KASP molecular marker and its application solve the problem that the excellent disease resistance alleles in wheat wild relatives are difficult to use in wheat breeding due to linkage burden

Method used

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  • KASP molecular marker co-segregated with powdery mildew resistant gene Pm58 and application of KASP molecular marker
  • KASP molecular marker co-segregated with powdery mildew resistant gene Pm58 and application of KASP molecular marker
  • KASP molecular marker co-segregated with powdery mildew resistant gene Pm58 and application of KASP molecular marker

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1 Powdery Mildew Resistance Gene of Knott wheat Pm58 Acquisition of co-segregated molecular markers

[0046] (1) (T093×TA1662) F 2 group building and Pm58 Screening for segmental recombinants

[0047] (1) The powdery mildew-susceptible parent T093 (♀) was crossed with the powdery mildew-resistant parent TA1662 (♂) to obtain the hybrid F 1 , F 1 Selfing produced F containing 676 individuals 2 group;

[0048] (2) Extract F by CTAB method 2 The DNA of each individual plant in the population; using Pm58 border markers Xhnu670 and Xhnu186 from F 2 Thirteen individual plants recombined in this segment were screened out in the population.

[0049] (2) 13 F's 2 Identification of Powdery Mildew Resistance in Selfed Progenies of Generational Recombinants

[0050] Powdery mildew resistance identification was carried out in the plant growth box, from each F 2 Randomly select 25 seeds from the self-bred progeny of the generation recombinant, and plant them in...

Embodiment 2

[0060] Example 2 Powdery Mildew Resistance Gene of Knott wheat Pm58 Application of co-segregated molecular markers

[0061] The resistance to powdery mildew was identified on a germplasm population containing 178 accessions of Triticum japonicus and 158 accessions of common wheat. The identification was carried out in a plant growth chamber, and the identification method was the same as in Example 1. Genomic DNA was extracted from all materials by the CTAB method, and Xkasp68500 Perform PCR amplification. The amplification method of KASP marker is the same as that in Example 1. Use LightCycler 480 software v1.5.1.62 software to analyze the fluorescence signal value of the amplified product, and record the results.

[0062] The results showed that only 5 of the 178 wheat germplasm materials carried the powdery mildew resistance allele consistent with TA1662, and these 5 materials were all resistant to powdery mildew strain E09. However, among the 158 accessions of common ...

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Abstract

The invention belongs to the field of crop breeding science, and relates to a KASP molecular marker co-segregated with a powdery mildew resistance gene Pm58 and application of the KASP molecular marker. And the genetic distance between the KASP marker and the Pm58 gene is 0 cM. The molecular marker most closely linked with Pm58 is obtained internationally for the first time. Xkasp68500 is a co-dominant marker and has the advantages of high throughput, high efficiency, stable amplification, convenience in detection and the like. The marker Xkasp68500 is used for detecting the Pm58 gene, whether the Pm58 exists or not and the existence state of the Pm58 can be determined, and then plants carrying the Pm58 are rapidly screened out and used for breeding new powdery mildew resistant wheat varieties.

Description

technical field [0001] The invention belongs to the field of crop breeding and relates to a powdery mildew resistance gene Pm58 Co-isolated KASP molecular markers and their applications. Background technique [0002] Wheat is one of the most important food crops in the world, providing about 20% of the energy for the global population. gramineous powdery mildew wheat specification ( Blumeria graminis f. sp. tritici, Bgt ) caused wheat powdery mildew to a great extent endangered the production of wheat. After wheat is infected with powdery mildew, the rate of photosynthesis decreases, and the number of tillers, grains per ear, and thousand-grain weight decrease, which affects the yield of wheat. In severe cases, the yield of wheat can be reduced by more than 30% (Singh et al. 2016). Compared with spraying chemical agents, breeding and planting disease-resistant varieties is one of the most cost-effective and sustainable means to solve the damage of wheat powdery mildew (...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2535/125C12Q2563/107Y02A50/30
Inventor 薛树林王新天王映雪孙铁鹏马钰彧
Owner HENAN UNIVERSITY
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