KASP marker for wheat powdery mildew resistance gene PmDTM and application of KASP marker

A powdery mildew resistance gene and powdery mildew resistance technology, which is applied in the field of KASP marker of wheat powdery mildew resistance gene PmDTM, can solve the problems of large import section and linkage burden, achieve convenient detection, stable amplification, and reduce breeding costs Effect

Active Publication Date: 2020-03-27
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention proposes a wheat powdery mildew resistance gene PmDTM The KASP marker and its application solved the problem of linkage redundancy caused by too large imported segment in wheat backcross breeding

Method used

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  • KASP marker for wheat powdery mildew resistance gene PmDTM and application of KASP marker
  • KASP marker for wheat powdery mildew resistance gene PmDTM and application of KASP marker
  • KASP marker for wheat powdery mildew resistance gene PmDTM and application of KASP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Wheat Powdery Mildew Resistance Gene PmDTM Acquisition of co-segregated molecular markers

[0043] (1) (Big head wheat × Huixian red) F 2 group building and PmDTM Screening for segmental recombinants

[0044] (1) The powdery mildew-resistant parent, barley (♀), was crossed with the powdery mildew-susceptible parent, Huixianhong (♂), to obtain the hybrid F 1 , F 1 Selfing produced F containing 1,192 individuals 2 group;

[0045] (2) Extract F by SDS method 2 The DNA of each individual plant in the population; using PmDTM The boundary markers GWM337 and BARC229 (http: / / wheat.pw.usda.gov / GG2 / index.shtml) from F 2 173 individuals recombined in this segment were screened out in the population.

[0046] (2) 173 F 2 Identification of Powdery Mildew Resistance in Selfed Progenies of Generational Recombinants

[0047] Powdery mildew resistance identification was carried out in the plant growth box, from each F 2 Randomly select 25 seeds from the self-crosse...

Embodiment 2

[0057] Example 2 Wheat Powdery Mildew Resistance Gene PmDTM Application of co-segregated molecular markers

[0058] Identification of resistance to powdery mildew was carried out on a core population of wheat containing 428 accessions. The identification was carried out in a plant growth chamber, and the identification method was the same as in Example 1. Genomic DNA of 428 materials was extracted by SDS method, and PCR amplification was performed with HNU58900. The amplification method of KASP marker is the same as that in Example 1. Use LightCycler 480 software v1.5.1.62 software to analyze the fluorescence signal value of the amplified product, and record the results.

[0059] The results showed that among the 428 wheat core germplasm materials, only 2 materials carried the same powdery mildew resistance allele as that of barley, and both materials showed broad-spectrum resistance to powdery mildew. This shows that the KASP marker HNU58900 provided by the present invent...

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Abstract

The invention belongs to the field of crop breeding science, and relates to a KASP marker for a wheat powdery mildew resistance gene PmDTM and an application of the KASP marker. The genetic distance between the KASP marker HNU58900 and the gene PmDTM is 0 cM. According to the invention, a molecular marker most closely linked with PmDTM is obtained internationally for the first time. The KASP marker HNU58900 is a codominant marker, and has the advantages of high throughput, high efficiency, stable amplification, convenient detection and the like. When the marker HNU58900 is used for detecting the gene PmDTM, whether PmDTM exists or not and the existence state of PmDTM can be determined; the powdery mildew resistance of wheat is predicted; and plants carrying PmDTM are rapidly screened and used for breeding new powdery mildew resistance varieties.

Description

technical field [0001] The invention belongs to the field of crop breeding and relates to wheat powdery mildew resistance gene PmDTM The KASP mark and its application. Background technique [0002] Wheat is the second largest food crop in the world after rice. In 2050, the total population of the world is expected to exceed 9 billion, and wheat production needs to maintain an average annual growth rate of 1.6% to meet the needs of population growth. Therefore, wheat production needs to increase from the current 3 tons / ha to 5 tons / ha by 2050 (Singh et al. 2016). by powdery mildew brucelli ( Blumeria graminis f. sp. tritici, Bgt ) caused by powdery mildew is a serious threat to the realization of this yield increase goal. Powdery mildew is a worldwide disease that occurs in wheat-growing areas all over the world, especially in high-yielding wheat-producing areas with a suitable climate. After powdery mildew infects wheat, it competes for the nutrition of the host an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 薛树林仇霄龙胡闪闪许红星
Owner HENAN UNIVERSITY
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