Method for evaluating curative effect of medication to cure HIV through detecting change of TCR gene of human T cell receptor before and after treatment

A cell receptor and gene technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as low sensitivity and specificity, and achieve highly reproducible results

Inactive Publication Date: 2004-03-10
CELLULAR BIOMEDICINE GRP SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The sensitivity and specificity of this quantitative method are low, and a large number of T cells (1×10 7 cell)

Method used

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  • Method for evaluating curative effect of medication to cure HIV through detecting change of TCR gene of human T cell receptor before and after treatment
  • Method for evaluating curative effect of medication to cure HIV through detecting change of TCR gene of human T cell receptor before and after treatment
  • Method for evaluating curative effect of medication to cure HIV through detecting change of TCR gene of human T cell receptor before and after treatment

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Embodiment Construction

[0029] Elaborate the specific implementation mode of the present invention below in conjunction with embodiment:

[0030] Blood samples from patients receiving AIDS drug treatment and healthy volunteers were collected separately, separated with Fico11-Hypaque layering fluid, and after horizontal centrifugation, mononuclear cells were densely concentrated in the interface between the plasma layer and the layering fluid, and the cells in this layer were aspirated After being washed and resuspended, PBMCs were obtained. CD4 or CD8 T cells were further isolated with magnetized beads coupled with CD4 or CD8 antibody.

[0031] The method for isolating and purifying the mRNA of T cells is to adopt the published technology (Chen, Z.W et al. 1994 proc, Matl. Acai. Sci. Usa. 91: 7501-7505), mRNA with 1 μg arbitrary hexanucleotides (Promega, the United States Madison, Wisconsin), 5 μl supertranscriptase II (Gibco BRL, Long Island, New York, USA) with a total volume of 20 μl, mixed at 37...

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Abstract

A method for evaluating the curative effect of the medicine to treat HIV infection features that a particular primer is designed to complete PCR in order to amplify the variation of T cell receptor (TCR) gene of HIV infected patient before and after the medicine is applied. Its advantages are high sensitivity and high correctness.

Description

Technical field: [0001] The invention relates to a method for evaluating the curative effect of drugs, in particular to a method for evaluating the curative effect of drugs for treating HIV by detecting changes in human T cell receptor TCR genes before and after treatment. Background technique: [0002] Human immunodeficiency virus (HIV) is a T-cell-tropic virus that infects target cells of CD4 T lymphocytes, thereby causing AIDS (AIDS). HIV infection can cause a reduction in the number of CD4 T cells and a disorder of immune function, as well as a high activation and functional disorder of CD8 T lymphocytes. Effective drug treatment can not only control the replication of the virus but also effectively improve or restore the number and function of T cells. Detecting the number and functional status of T cells is the most powerful biological and immunological basis for identifying the efficacy of drugs. [0003] At present, the commonly used method is to use flow cytometry...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 寇忠琛
Owner CELLULAR BIOMEDICINE GRP SHANGHAI
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