Stem inductive method for microbody reproduction of Japan dahurian larch

A technology of micropropagation and larch, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of Japanese larch micropropagation without relevant reports, only focusing on scientific issues, and low reproductive rate, and achieve Improve the effect of tissue branching and rooting rate, fast reproduction speed and high reproduction efficiency

Inactive Publication Date: 2004-04-28
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, although the continuous micropropagation system has been established in the sapling micropropagation of European larch, they may have a low reproductive rate, such as Itahana has utilized the top bud of Japanese larch (document 8: N.Itahana, 1995, Manual for budculture of Japanese larch, Bulletin of the National Forest Tree Breeding Center, 13:145-149), w

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Stem inductive method for microbody reproduction of Japan dahurian larch
  • Stem inductive method for microbody reproduction of Japan dahurian larch
  • Stem inductive method for microbody reproduction of Japan dahurian larch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Select the clonal plants of Japanese larch, use the 1-year-old branch explants before bud germination in early spring as the material, take the axillary buds, adventitious stem formation, elongation induction, and subculture to realize the regeneration of stem organs; specific operations as follows:

[0034] 1. Material collection and pretreatment

[0035] The Japanese larch Chao No. 85 clone was selected from the seed garden of Wulong Forest Farm, Fushun County, Liaoning Province. The tree was 12 years old, and the middle branches of the 1-year-old tree crown were collected. The collection time was before the buds germinated in early spring. At this time, the bud scales were formed from dull Dark brown or dark brown turns into shiny light brown or light brown. Store at 4°C. After taking it out, cultivate it in water at room temperature for two weeks until the buds are bright, and set aside.

[0036] 2. Preparation of Media

[0037] 1) prepare conventional WPM medium,...

Embodiment 2

[0053] The difference from Example 1 is:

[0054] 1) Select the 28-year-old Japanese larch clone No. 38 plant, and collect 1-year-old branches full of winter buds in March as materials;

[0055] 2) Prepare the culture medium SH, the medium composition is: KNO 3 2500mg / l, NH 4 h 2 PO 3 300mg / l, CaCl 2 2H 2 O 200mg / l, MgSO 4 ·7H 2 O 400mg / l, FeSO 4 ·7H 2 O 15.0mg / l, Na 2 EDTA·2H 2 O 20.0mg / l, H 3 BO 3 5.0mg / l, ZnSO 4 ·7H 2 O 6.0mg / l, MnSO 4 4H 2 O10.0mg / l, Na 2 MoO 4 2H 2 O 0.1mg / l, KI 1.0mg / l, CoCl 2 ·6H 2 O 0.1mg / l, inositol 1000mg / l, thiamine hydrochloride 5.0mg / l, niacin 5.0mg / l, pyridoxine hydrochloride 0.5mg / l, agar 6000mg / l.

[0056] 3) Inoculation of axillary buds

[0057]Add plant growth regulating substances in the SH medium: Zea 0.2mg / l, IAA 0.5mg / l, transfer the sterilized axillary buds to the prepared Erlenmeyer flasks equipped with the SH medium, every Connect 3 to 6 axillary buds in the bottle to induce the formation of adventitious stem...

Embodiment 3

[0066] Repeat the test of embodiment 1 and embodiment 2, culture medium and culture condition are with above-mentioned respectively, the additional growth regulating substance is BA 0.1mg / l or Zea 0.01mg / l or NAA0.01mg / l in the stem induction stage, in During the stem elongation stage, IBA 0.3mg / l, NAA 0.1mg / l and AC 10g / l were taken at the beginning of April. Among the 181 axillary buds tested, 80.06% of the axillary buds completed the regeneration of stem organs. Fig. 3A, 3B, 3C, 3D show the result of above-mentioned two embodiment repeated tests, wherein Fig. 3A is the rooting culture medium of present embodiment 1, Fig. 3B and 3C are verification result of embodiment 1, Fig. 3D is present embodiment 2 verification results.

[0067] Fig. 3 is the adventitious stem in this example, indicating that the appropriate dosage of BA or Zea or NAA in the present invention alone can also complete the induction of stem (Fig. 3A) and complete the regeneration of stem organ (Fig. 3B). ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to forest reproduction and plant regeneration technology in forestry science, and is especially stem inducing method for microbody reproduction of Japanese larch. From annual branch of Japanese larch of different age, the axillary bud as the clone material is taken and processed through adventitious stem formation, growth, subculture and other steps to reproduce stem organ. The present invention provides one reproduction method with fast speed, short period, no influence of seasoning and high multiplication efficiency for the formation of the reproduction plant of Japanese larch.

Description

technical field [0001] The invention relates to tree propagation and plant regeneration technology in forestry science, in particular to a stem induction method for Japanese larch micropropagation. Background technique [0002] Larix is ​​a subfamily of Laricodeae in the family Pinaceae. This genus is rich in germplasm resources. According to data reports, there are 25 species of Larix and several varieties and hybrids in the world, of which 17 species are distributed in my country ( Document 1: Wang Zhan (editor-in-chief), Zhang Songyun (deputy editor-in-chief), 1992, Chinese larch forest, Beijing: China Forestry Press, pp1-3). They are widely distributed throughout the subtropical mountains, temperate mountains and cold temperate plains in the northern hemisphere. Larix has excellent qualities such as strong adaptability, fast growth, early forest formation, resistance to diseases and insect pests, high wood strength, and strong corrosion resistance. It has a wide range of...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H4/00
Inventor 王力华贺凤美许思明
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap