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Trichoderma harziarum strain

A technology of strains and fungi, applied in the field of microorganisms, can solve the problems of limited application range, great difference in disease prevention effect, and small spore production

Inactive Publication Date: 2004-12-15
何月秋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is precisely because of the wide range of sources of strains that the application conditions are strict in use, and each strain has its own unique scope of action, that is to say, their effect of preventing and controlling diseases has obvious ecological dependence, that is, depending on atmospheric environmental conditions (including Temperature, humidity, rainy days, rainfall, atmospheric pressure, light, ultraviolet intensity, etc.), crop objects (including crop types, diseased parts, developmental stages, growth postures, composition of exocrine substances, and other types of microorganisms on the surface or inside of crops, etc.) , Different soil conditions (including temperature, humidity, physical and chemical properties, organic matter content, other biological population structures, etc.), the effect of disease prevention varies greatly
Existing Trichoderma strains have a small amount of spore production, and there are not many strains that are efficient, stable and suitable for industrial production, and each strain can generally only be used for the inhibition of a few plant pathogens, and the scope of use is very limited

Method used

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  • Trichoderma harziarum strain
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Embodiment Construction

[0012] First, Trichoderma harzianum Rifai strain B (hereinafter referred to as Th-B) was isolated from soil in Dounan greenhouse, Chenggong County, Kunming City, Yunnan Province, and cultivated on PSA solid medium at 28°C. Fungal strains, it was observed that the strains grew very fast. The fungal strain can be cultivated in two ways, one is the flat plate method, that is, the conidia of the bacterial strain are diluted to 50-100 conidia under the microscope field of 100 times, and the conidia are spread on a PSA plate and placed at 20°C. A large number of conidia can be harvested until the 5th day of cultivation; the other is the semi-liquid and semi-solid method, that is, the conidia liquid is put into PS liquid medium, shaken for 3-4 days, and then press 1:5 Mix ~8 ratio with sterilized rice bran or wheat bran, cover with 70% alcohol sterilized plastic film, place in a sterilized room at 23-28°C for 3-4 days, open the plastic film, and produce spores 3-5 days; together wit...

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Abstract

The present invention relates to a kind of Trichoderma harzianum strain, belongs to the field of microbiological technology. The Trichoderma harzianum strain, named as Trichoderma harzianum B, is preserved in the preservation number of CGMCC No. 0955, and belongs to Moniliaaceae, Hyphomycetales, Hyphomycetidae of Deuteromycotina. The Trichoderma harzianum strain of the present invention has high spore yield and high efficiency wide spectrum plant disease preventing capacity, and may be used to promote plant growth and suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of microbes, and mainly relates to a Trichoderma harzianum fungus strain capable of broad-spectrum inhibiting plant diseases and promoting plant growth. Background technique [0002] Today, the calls for the development of sustainable agriculture are getting higher and higher, and the demand for green products is increasing day by day. Due to their short efficacy time, easy to cause environmental pollution and residues on plants, the use of chemical pesticides is gradually reduced. For this reason, many people are devoting themselves to the research of plant biocontrol technology that does not utilize chemical methods and does not cause pollution. With the maturity of biological control technology, Trichoderma, as a class of antagonistic microorganisms rich in resources, plays an increasingly important role in the biocontrol of plant diseases. Trichoderma is a type of fungus with a very wide distribution ra...

Claims

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Application Information

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IPC IPC(8): C12N1/15
Inventor 何月秋张乃明李作森杨合同
Owner 何月秋
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