Microbe method for preparing enamine and amine from valinemia

A technology of mycylamine and microorganisms, applied in the field of Stenotrophomonas maltophilia, can solve the problems of inapplicability of effective mycylamine and mass production of effective mycylamine, and weak decomposition ability of effective mycin

Active Publication Date: 2005-01-12
ZHEJIANG UNIV OF TECH +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] There have been more than 30 years of research on the production methods of effective mycosylamine and effective mycosylamine. Japanese Kameda et al. once used the bacteria of Pseudomonas denitrification to degrade effective mycomycin A or effective mycoylidene amine A , and then separate

Method used

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  • Microbe method for preparing enamine and amine from valinemia
  • Microbe method for preparing enamine and amine from valinemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Culture medium formula (weight / volume percentage, the same below): validamycin: 1.0%, (NH 4 ) 2 SO 4 : 1.0%, KCl: 0.5%, Na 2 HPO 4 12H 2 O: 1.0%, NaH 2 PO 4 2H 2 O: 0.1%, MgSO 4 : 0.01%, prepared with tap water, and adjusted the pH to 6.0 with HCl solution.

[0056] Take 100mL of the above-mentioned culture medium, divide it equally into two 250mL Erlenmeyer flasks, and sterilize. Inoculate the slant strain CCTCC No.M 204024, cultivate the bacteria, shake the table at a speed of 150r / min, and cultivate it at 28°C for 72 hours as a seed solution for later use.

[0057] Take 2L of the above culture medium, divide it into 20 500mL shake flasks, and sterilize. The seed liquid was inserted, the inoculum amount was 2% (v / v), and culture was carried out, the culture temperature was 20° C., the culture time was 160 h, and the shaker speed was 200 r / min.

[0058] Collect 1.8L of the above-mentioned fermentation broth, centrifuge to remove the bacteria, and the superna...

Embodiment 2

[0060] Medium formula Effectivemycin: 17.0%, (NH 4 ) 2 SO 4 : 8.0%, KCl: 0.5%, Na 2 HPO 4 12H 2 O: 1.0%, NaH 2 PO 4 2H 2 O: 0.1%, MgSO 4 : 0.01%, prepared with tap water, and adjusted the pH to 7.0 with NaOH solution.

[0061] Take 500mL of culture medium, evenly distribute it in five 500mL Erlenmeyer flasks, and sterilize. The slant strain CCTCC No.M 204024 was inserted and cultured at 28°C for 150 hours. The reaction was carried out under the conditions of stirring, ventilation and shaking, and the speed of the shaker was 200r / min.

[0062] 400 mL of the above-mentioned fermentation broth was collected, separated and purified, and the steps were the same as in Example 1 to obtain 0.56 g of available mycylamine and 0.85 g of available mycylamine.

Embodiment 3

[0064] Fermentation medium formula validamycin: 8.0%, (NH 4 ) 2 SO 4 : 6%, KCl: 0.1%, Na 2 HPO 4 12H 2 O: 1.0%, NaH 2 PO 4 2H 2 O: 0.16%, MgSO 4 : 0.02%, prepared with tap water, and adjusted the pH to 7.5 with NaOH solution.

[0065] Seed medium formula effectivemycin: 1.0%, (NH 4 ) 2 SO 4 : 1%, KCl: 0.5%, Na 2 HPO 4 12H 2 O: 1.0%, NaH 2 PO 4 2H 2 O: 0.1%, MgSO 4 : 0.5%, prepared with tap water, and adjusted the pH to 7.5 with NaOH solution.

[0066] Take 500mL seed culture medium, divide it into five 500mL Erlenmeyer flasks, and sterilize. Insert the slant strain CCTCC No.M 204024, cultivate the bacteria, shake the rotating speed of 200r / min, and cultivate in the shaking table at 30°C for 72 hours as the seed liquid, and set aside.

[0067] Take 8L of fermentation medium, divide it into 80 shake flasks of 500mL, and sterilize. Insert the seed solution, the inoculation amount is 5% (v / v), and cultivate, the cultivation temperature is 30°C, the cultivation...

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Abstract

A microorganism preparation method relates to the microorganism of stenotrophomonas maltrophilia of stenotrophomonas utilized by CCTCC NoM 204024 of its fermentation and decomposition or its cells or zymes decomposite substrates validamycin or validamycin imide to produce validamy enamine and validemy amine. Composition of the culture media is(weight/volume): validamycin 0.5-20.0%,(NH4)2 SO4 0.5%-10.0, KCL 0.5%-5.0%, Na2HPO4, 12H2 00.1%-10.0%, Na2H2PO4 2H2O 0.1-5.0%, MgSO4 0.01%-1.0% matched by running water, fermenting temperature: 20deg.C-40-deg.C initial PH:6.0-8.0 for 1-180h by ionic exchanges, chromatography and purify, the two products are finished.

Description

technical field [0001] The present invention relates to the new stenotrophomonas maltrophilia (Stenotrophomonas maltrophilia) screened from the soil, and also relates to utilizing the new bacterial strain to decompose effective mycomycin (also known as Jinggangmycin) or effective mycoylidene amine (also known as Jinggang A method for producing effective mycylamine and effective mycylamine. Background technique [0002] There have been more than 30 years of research on the production methods of effective mycosylamine and effective mycosylamine. Japanese Kameda et al. once used the bacteria of Pseudomonas denitrification to degrade effective mycomycin A or effective mycoylidene amine A , and then separated to obtain effective mycamine and effective mycamine, but they cannot grow on a medium with only effective mycin as the sole carbon source; and the decomposition ability of effective mycin is very weak, so it is not suitable for effective myc Mass produc...

Claims

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Application Information

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IPC IPC(8): C12P13/00
CPCC12P13/001
Inventor 郑裕国陈小龙薛亚平王远山沈寅初
Owner ZHEJIANG UNIV OF TECH
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