Simple extraneous liver perfusion device for digesting and separating liver cells

Abstract

A simple in vitro liver perfusion apparatus for digesting and separating liver cell characterized in that a container is installed on the engine block, inside the container being positioned a stainless reticular baffle plate, a heating device being installed in the container bottom under the stainless reticular baffle plate, a perfusion bottle with a side hole is fixed on the top surface of stainless reticular baffle plate by a fixing clamp, and a stainless filter net is fixed on the top part by bracket. An input end of peristaltic pump on the engine block is connected with an aseptic input conduct, while the out put end of the peristaltic pump is connected with an aseptic outputting conduct, needle handle and perfusion needle. It can realize two step liver perfusion for in vitro liver, obtain effective perfusion for anterior perfusion fluid and digestive fluid rapidly and continuously in the largest extent, and monitor liver tissue process

Description

(1) Technical field [0001] The invention relates to medical experiment equipment and auxiliary equipment of medical instruments, in particular to an extracorporeal liver perfusion device for the purpose of obtaining liver cells. (2) Technical background [0002] The existing mature hepatocyte separation method is the enzymatic digestion method, in which collagenase etc. are injected into the liver blood vessels to act on the liver tissue to dissolve the complex fibrous connections between the hepatocytes, thereby obtaining complete individual hepatocytes. Due to the lack of unified standards, methods and instruments, it is difficult for laboratories and experimenters to master this technology, resulting in different separation effects and low cell acquisition rate and viability. In the enzymatic digestion method, no matter in situ or in vitro perfusion, manual syringe injection is generally used, and 20ml of perfusate is drawn each time for repeated perfusion. This method i...

AI Technical Summary

Problems solved by technology

At the same time, the perfusion pressure of the manual injection method is not uniformly controlled. When the pressure is too low, the perfusion is insufficient, and when the pressure is too high, it will cause damage to the cells.

Since collagenase has a high requirement on temperature, 37°C is its optimum temperature, and it is difficult to control the temperature of the perfusate by the above method. Therefore, the final cell digestion effect is often affected due to poor temperature control, and the desired cells cannot be obtained. Quantity and quality requirements, and cause waste of expensive collagenase, increasing the cost of isolating and culturing hepatocytes

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