Use of protein fingerprint method in detecting protein fingerprint of pancreas cancer cell in blood

A protein and protein technology, applied in the field of protein fingerprinting and protein fingerprinting of biological sample analysis, can solve the problems of inability to observe molecular weight, inability to detect biomolecules, complexity, etc.

Inactive Publication Date: 2005-03-16
BEIJING SELDI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the method provides only two characteristics of biomolecules - mass and isoelectric point (pI)
Second, the resolution in each dimension is limited by the resolving power of the gel
For example, it is often difficult to distinguish between biomolecules that differ by less than 5% in mass or that differ in pI
Third, gels have limited capacity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Differentiation of protein fingerprints in blood between normal human cells and pancreatic cancer patients

[0064] (1) Experimental method

[0065] 1. Material

[0066] 1. Specimen source: There are 33 cases in the pancreatic cancer patient group, all of which were pathologically diagnosed as pancreatic cancer. There are 33 cases in the normal group (healthy volunteers with normal health checkup).

[0067] 2. Reagents: Urea, acetonitrile, trifluoroacetic acid, and SPA (Sinapinic acid) were all purchased from Sigma.

[0068] Two, method

[0069] 1. Sample collection: After collecting whole blood, draw serum and store at -80℃;

[0070] 2. Sample preparation: Take 20μl of serum and dilute it to 150μl with pH7.0 buffer (100mmol / L sodium phosphate, 250mmol / L sodium chloride) according to 1:7.

[0071] 3. Chip pretreatment: Coordinated covalent metal chelating agent acts as adsorbent chip. Add 50μl of 100mmol / L copper sulfate to each hole, shake for 5min at room temper...

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PUM

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Abstract

This invention also relates to protein dactylography method for analyzing for the protein group got by retention chromatography. This method adopts mass spectroscopy to distinguish and detect protein group. The analysis data takes the form of bar code (protein dactylography) read by computer. This invention method can be used to detect protein fingerprint in the normal cell and cancer of stomach patient cell in the blood.

Description

Technical field [0001] The invention relates to a new method for analyzing protein in biological samples, and specifically refers to a protein fingerprint method for analyzing biological samples. The invention also relates to a protein fingerprint method that captures the proteome by retention chromatography and uses a computer-readable barcode format (protein fingerprint) for analysis. Background technique [0002] Both the normal functions and pathological characteristics of cells depend to a certain extent on the functions of proteins expressed by cells. Therefore, the identification of differences in proteins expressed in cells can be used to diagnose diseases, and ultimately for drug development and disease treatment. The differential analysis of protein expression and function requires the ability to distinguish complex mixtures of intracellular molecules. However, many substances in cells are often present in trace amounts. The current methods for protein analysis have lim...

Claims

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Application Information

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IPC IPC(8): G01N30/00G01N30/72G01N33/53G01N33/68G06K7/10H01J49/04
Inventor 许洋陈国强
Owner BEIJING SELDI TECH
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