Quality detection method for glass micro flow control chip
A quality detection method, glass micro technology, applied in the direction of analysis materials, instruments, etc., can solve the problem of inability to comprehensively and accurately reflect the physical and chemical properties of microfluidic chips, the lack of uniformity of quality detection methods for microfluidic chips, and lack of research. Improvement and other issues
Inactive Publication Date: 2005-06-29
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology
However, microfluidic chips have many characteristics different from traditional capillary electrophoresis due to their relatively small channel size and variable channel design, especially the injection channel and separation channel, although the academic community has noticed this difference , but on the whole, the research on this aspect is still very imperfect (AlarieJ.P., Jacobson S.C., Culbertson C.T. et al.. Electrophoresis[J], 2000, 21:100-106. Jacobson S.C., Hergenroder R ., Koutny L.B. et al.. Anal. Chem. [J], 1994, 66: 1107-1113.)
In particular, there is still a lack of uniform standards for the quality detection methods of microfluidic chips. The literature (Indalesio R., Paolo Spicar-Mihalic, et al Analtica ChimicaActa[J], 2003, 496: 205-215) monitors the chip by profilometer The profile of the channel, the smoothness of the inner wall of the channel is detected by scanning electron microscopy; literature (Jimmy D.C., Mark S.C., et al.Biomaterials[J], 2002, 23:929-935.) uses electron microscopy to scan the microfluidic chip channel
The detection method is relatively simple and cannot comprehensively and accurately reflect the physical and chemical properties of the microfluidic chip
Method used
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Embodiment 1
[0019] The sampling length of the profiler is between 0.2mm and 0.5mm, the evaluation length is between 0.4mm and 0.8mm, the measuring speed is 1mm / s, the vertical magnification is 1000, the scale is 10μm / grid; the horizontal magnification is 200, the scale is 50 μm / grid, and the profile curve of the channel cross-section of the glass microfluidic chip is measured as figure 1 shown.
Embodiment 2
[0021] The scanning electron microscope images of the cross channel and the separation channel of the glass microfluidic chip are as follows: figure 2 As shown, the magnifications are 100 and 400, respectively.
Embodiment 3
[0023] Use phosphate buffer with a pH value between 3 and 11 and a concentration between 5 and 20mM as the electrophoresis buffer, and add an equal amount of different concentrations of electrophoresis buffer to the reservoirs at both ends of the separation channel of the chip. The difference should be above 5%. To measure the electroosmotic value in the microchannel at different pH, see the attached image 3 .
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Abstract
This invention provides a quality monitor method of glass micro flow control chip, which is characterized by the flowing: the micro control chip section outline measurement; surface finishment measurement; surface discharge distribution measurement and chemical property identification. The advantages of this invention lie in the following: first to monitor the product quality of the glass micro control chip; to give an ideal method to test the products quality from different factories.
Description
Technical field: [0001] The invention relates to profilometer, scanning electron microscope, electroosmotic flow measurement method and relative standard deviation measurement technology of migration time, and particularly provides a quality detection method of glass microfluidic chip. Background technique: [0002] In the early 1990s, Manz et al. proposed the concept of "Micro-total analysis system" (Micro-total analysis system, μ-TAS) (Harrison D.J., Fluri K., Seiler K. et al..Science[J], 1993, 261 : 895-897), and successfully transplanted the traditional capillary electrophoresis onto the glass chip. Since then, various microfluidic chips have attracted extensive attention and developed rapidly. At present, glass and quartz are the most used chip materials (Fan A.H., Harrison D.J., Anal.Chem.[J], 1994, 66:177-184; Koutny L.B., Schmalzing D., Taylor T.A.et al..Anal.Chem. [J], 1996, 68: 18-22.). [0003] The basic commonality between microfluidic chips and traditional cap...
Claims
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IPC IPC(8): G01N35/00
Inventor 王辉戴忠鹏王利白吉玲林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI