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Large-scale seaweed cell or tissue reactor pre-culturing system

A cell culture and reactor technology, applied in the field of pre-culture systems, can solve the problems of dispersion, one-sidedness, and singleness, and achieve the effects of reliable experimental data, reduction of time and cycle, and reduction of system errors and human errors.

Inactive Publication Date: 2005-07-06
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art, aiming at the scattered, single, one-sided and cumbersome defects when exploring various culture conditions and engineering parameters in the pre-cultivation stage, and to provide a pre-cultivation system for large seaweed cells or tissue reactors

Method used

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  • Large-scale seaweed cell or tissue reactor pre-culturing system
  • Large-scale seaweed cell or tissue reactor pre-culturing system
  • Large-scale seaweed cell or tissue reactor pre-culturing system

Examples

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Embodiment Construction

[0023] Such as Figure 1-6 As shown, the present invention includes: an external air supply unit 1, a filter sterilizer 2, a gas humidifier 3, an ethanol steam generator 4, a constant temperature incubator 5, a three-way valve 6, an internal multi-channel gas distribution unit 7, and Mobile lighting system 8, cell culture unit 9. Its connection mode is: external air supply unit 1, filter sterilizer 2 and ethanol steam generator 4 are set outside the constant temperature incubator 5, gas humidification device 3, three-way valve 6, internal multi-channel gas distribution unit 7, can The mobile lighting system 8 and the cell culture unit 9 are set in the constant temperature incubator 5; the main body of the gas humidification device 3 is a cylindrical glass container with a hemispherical top, the top of which is integrated with an air inlet port 10 and an air outlet port 11, and the air inlet port 10 extends into the container near the bottom. The external air supply unit 1, t...

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Abstract

A pre-culturing system of large-scale seaweed cell or tissue reactor comprises an outer gas supply unit, a filtering sterilizer and an ethanol steam generator arranged outside the constant temperature incubator, a gas humidifying device, a three-way valve, a gas distribution unit with inner multi-channels, a moveable illuminating system, and a cell culture unit arranged inside the constant temperature incubator, wherein the gas humidifying device is provided with an inlet interface and an outlet interface on the top, the inlet interface extends into the near bottom of the container, the inlet interfaces of the outer gas supply unit, the filtering sterilizer and the gas humidifying device sequently connect with each other via silica gel gas transmission tubes, the three ends of the three-way valve respectively connect with the outlet interface of the gas humidifying device, the ethanol steam generator and the gas distribution unit with inner multi-channels via silica gel gas transmission tubes, the moveable illuminating system is arranged outside the cell culture unit to compose a whole set assembly with the cell culture unit, the gas distribution unit with inner multi-channels has multiple ends, each end connects with the cell culture unit via the silica gel gas transmission tube.

Description

technical field [0001] The invention relates to a precultivation system, in particular to a macroalgae cell or tissue reactor precultivation system. Used in marine engineering field. Background technique [0002] Many macroalgae contain important biologically active substances, but most of the active compounds are in very small amounts and have complex structures, so it is difficult to obtain them by direct extraction or artificial synthesis. The use of photobioreactors to cultivate macroalgae cells or tissues under controllable and engineering-optimized conditions is an effective means to obtain these active substances in industrialized large-scale production in the future, and bioreactor cultivation has the advantages of online detection, controllability, and uniform product quality. advantage. However, it is not easy to separate and establish clonal lines of macroalgae cells or tissues for culture, and the culture conditions are much more complex and harsh than those of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
Inventor 陈思晔齐瀚实张宝红赵锐强静
Owner SHANGHAI JIAO TONG UNIV
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