Method for ectracting general alkaloid of Chinese goldthread and evodia fruit and preparation

Abstract

A process for preparing the alkaloid from coptis root and evodia fruit, which can be used to prepare the analgesic for gastritis and gastric ulcer, includes such steps as pulverizing, mixing, reflux extraction in alcohol, recovering alcohol, extracting by ethyl acetate, recovering ethyl acetate to obtain extract A, decocting the dregs, filtering, concentrating, mixing, filter regulating pH=1-4, adding NaCl, stirring, laying aside, filtering, washing deposit by water to obtain extract B, mixing A with B, vacuum drying and pulverizing.

Description

Technical field

[0001] The invention relates to a method for extracting effective components of Chinese medicine compound Coptis and Evodia and the application of the extracts, in particular to a method for extracting total alkaloids from compound Chinese medicines. Background technique

[0002] The total alkaloids of Rhizoma Coptidis and Evodia have good curative effect on acute and chronic gastritis, enteritis, gastric ulcer and other diseases. However, in the production process of Coptis and Evodia, the raw material is extracted by decoction (or directly crushed to make finished medicine). The extract obtained by this method is an extract, which has low purity and hygroscopicity. Strong, difficult to prepare preparations, poor quality of preparations and large dosage. A patent application for invention with publication number CN 1431014A has disclosed a method for extracting the total alkaloids of Coptis and Evodia. The method is: adding fixed formula powder of Coptis and Evod...

Examples

Embodiment 1

[0028] Example 1: Follow the steps below:

[0029] a. Preparation: Take 21kg of Coptis and Evodia 3.5kg as raw materials, pulverize Coptis and Evodia into coarse powder and mix to obtain powder;

[0030] b. Extraction: Add 196kg of 90% ethanol to the powder, extract twice, 1 hour each time, filter and combine the filtrate to obtain the ethanol extract; the medicine residue is decocted twice with 196kg water, 1 hour each time , Filter, combine the decoction liquid and concentrate to a relative density of 1.0~1.2 to obtain a concentrated liquid;

[0031] c. Recover ethanol from the ethanol extract of step b to obtain a thick paste, extract twice with 24500ml ethyl acetate, and recover the ethyl acetate to obtain extract A and aqueous solution; the aqueous solution and the concentrated solution of step b are combined, stand still, and filter The filtrate was adjusted to pH 2.0 with hydrochloric acid, NaCl was added to make the content 6% (weight / volume), stirred to dissolve, filtered...

Embodiment 2

[0034] Example 2: Follow the steps below:

[0035] a. Preparation: Take 21kg of Coptis and Evodia 3.5kg as raw materials, pulverize Coptis and Evodia into coarse powder and mix to obtain powder;

[0036] b. Extraction: Add 147kg of 70% ethanol to the powder, extract 2 times at reflux, 1 hour each time, filter and combine the filtrate to obtain the ethanol extract; the medicine residue is decocted twice with 147kg water, 1 hour each time , Filter, combine the decoction liquid and concentrate to a relative density of 1.0~1.2 to obtain a concentrated liquid;

[0037] c. Recover ethanol from the ethanol extract of step b to obtain a thick paste, extract twice with 49000ml ethyl acetate, and recover the ethyl acetate to obtain extract A and an aqueous solution; the aqueous solution and the concentrated solution of step b are combined, stand still, and filter , The filtrate was adjusted to pH 1.0 with hydrochloric acid, NaCl was added to make the content 5% (weight / volume), stirred to d...

Embodiment 3

[0040] Example 3: Follow the steps below.

[0041] a. Preparation: Take 21kg of Coptis and Evodia 3.5kg as raw materials, pulverize Coptis and Evodia into coarse powder and mix to obtain powder;

[0042] b. Extraction: Add 245kg of 95% ethanol to the powder, extract 2 times at reflux, 1 hour each time, filter, combine the filtrate to obtain the ethanol extract; the medicine residue is decocted twice with 245kg water, 1 hour each time , Filter, combine the decoction liquid and concentrate to a relative density of 1.0~1.2 to obtain a concentrated liquid;

[0043] c. Recover ethanol from the ethanol extract of step b to obtain a thick paste, extract twice with 98000 ml of ethyl acetate, and recover the ethyl acetate to obtain extract A and an aqueous solution; the aqueous solution and the concentrated solution of step b are combined, stand still, and filter The filtrate was adjusted to pH 4.0 with hydrochloric acid, NaCl was added to make the content 10% (weight / volume), stirred to d...

Technical field

[0001] The invention relates to a method for extracting effective components of Chinese medicine compound Coptis and Evodia and the application of the extracts, in particular to a method for extracting total alkaloids from compound Chinese medicines. Background technique

[0002] The total alkaloids of Rhizoma Coptidis and Evodia have good curative effect on acute and chronic gastritis, enteritis, gastric ulcer and other diseases. However, in the production process of Coptis and Evodia, the raw material is extracted by decoction (or directly crushed to make finished medicine). The extract obtained by this method is an extract, which has low purity and hygroscopicity. Strong, difficult to prepare preparations, poor quality of preparations and large dosage. A patent application for invention with publication number CN 1431014A has disclosed a method for extracting the total alkaloids of Coptis and Evodia. The method is: adding fixed formula powder of Coptis and Evod...

Examples

Embodiment 1

[0028] Example 1: Follow the steps below:

[0029] a. Preparation: Take 21kg of Coptis and Evodia 3.5kg as raw materials, pulverize Coptis and Evodia into coarse powder and mix to obtain powder;

[0030] b. Extraction: Add 196kg of 90% ethanol to the powder, extract twice, 1 hour each time, filter and combine the filtrate to obtain the ethanol extract; the medicine residue is decocted twice with 196kg water, 1 hour each time , Filter, combine the decoction liquid and concentrate to a relative density of 1.0~1.2 to obtain a concentrated liquid;

[0031] c. Recover ethanol from the ethanol extract of step b to obtain a thick paste, extract twice with 24500ml ethyl acetate, and recover the ethyl acetate to obtain extract A and aqueous solution; the aqueous solution and the concentrated solution of step b are combined, stand still, and filter The filtrate was adjusted to pH 2.0 with hydrochloric acid, NaCl was added to make the content 6% (weight / volume), stirred to dissolve, filtered...

Embodiment 2

[0034] Example 2: Follow the steps below:

[0035] a. Preparation: Take 21kg of Coptis and Evodia 3.5kg as raw materials, pulverize Coptis and Evodia into coarse powder and mix to obtain powder;

[0036] b. Extraction: Add 147kg of 70% ethanol to the powder, extract 2 times at reflux, 1 hour each time, filter and combine the filtrate to obtain the ethanol extract; the medicine residue is decocted twice with 147kg water, 1 hour each time , Filter, combine the decoction liquid and concentrate to a relative density of 1.0~1.2 to obtain a concentrated liquid;

[0037] c. Recover ethanol from the ethanol extract of step b to obtain a thick paste, extract twice with 49000ml ethyl acetate, and recover the ethyl acetate to obtain extract A and an aqueous solution; the aqueous solution and the concentrated solution of step b are combined, stand still, and filter , The filtrate was adjusted to pH 1.0 with hydrochloric acid, NaCl was added to make the content 5% (weight / volume), stirred to d...

Embodiment 3

[0040] Example 3: Follow the steps below.

[0041] a. Preparation: Take 21kg of Coptis and Evodia 3.5kg as raw materials, pulverize Coptis and Evodia into coarse powder and mix to obtain powder;

[0042] b. Extraction: Add 245kg of 95% ethanol to the powder, extract 2 times at reflux, 1 hour each time, filter, combine the filtrate to obtain the ethanol extract; the medicine residue is decocted twice with 245kg water, 1 hour each time , Filter, combine the decoction liquid and concentrate to a relative density of 1.0~1.2 to obtain a concentrated liquid;

[0043] c. Recover ethanol from the ethanol extract of step b to obtain a thick paste, extract twice with 98000 ml of ethyl acetate, and recover the ethyl acetate to obtain extract A and an aqueous solution; the aqueous solution and the concentrated solution of step b are combined, stand still, and filter The filtrate was adjusted to pH 4.0 with hydrochloric acid, NaCl was added to make the content 10% (weight / volume), stirred to d...