Droplet discharging device, apparatus for manufacturing microarray, and method for manufacturing microarray

Abstract

A small droplet discharging device is provided capable of producing high- density arrays. The droplet discharging device comprises a first substrate comprising a plurality of reservoir chambers for holding a liquid,supply openings supply of a liquid stored in the reservoir chambers, a second substrate comprising a plurality of discharge units comprising supply openings for receiving a supply of a liquid stored in the reservoir chambers, pressurizing chambers for applying a pressure to the liquid supplied from the supply openings, and discharge openings for discharging the liquid pressurized in the pressurizing chambers to the outside, and a third substrate sandwiched between the first substrate and the second substrate and comprising channels for connecting the plurality of reservoir chambers with the plurality of supply openings corresponding thereto, wherein the plurality of supply openings provided in the second substrate are arranged such that relative positions thereof have a zigzag disposition.

Description

Background technique [0001] The present invention relates to a droplet discharge device that discharges a very small amount of liquid, a microarray manufacturing device and a microarray manufacturing method using the droplet discharge device. [0002] In recent years, the interpretation of DNA base sequences and the functional analysis of gene information have become current issues, and DNA microarrays have been widely used as techniques for monitoring gene discovery patterns and screening for novel groups. The array is formed by modulating the probe DNA to form a spot on a substrate such as a glass slide at a high density, and hybridizing the target DNA with an auxiliary base sequence with the probe DNA among the fluorescently labeled target DNA, and observing the fluorescent pattern. To evaluate the amount of gene discovery. [0003] Using this technology, we have also developed a protein chip that attaches proteins to a substrate at a high density, and is used for protein ...

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Problems solved by technology

[0006] However, this droplet ejection device still has room for improvement in terms of miniaturization of the device due to the flow path having a multi-layer structure.

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