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Method for partitioning germ microspecies of bacterial leaf spot of paddy rice

A rice bacterial blight and pathogen technology, which is applied in the fields of rice cultivation, botanical equipment and methods, horticulture, etc., can solve the problems of unscientific, inconsistent genetic background of identification varieties, large randomness of races, etc., and achieves accurate survey data. Effect

Inactive Publication Date: 2005-11-16
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the genetic backgrounds of the identification varieties used are inconsistent, the disease resistance genes contained in them are not completely clear, and there are no strict requirements on the inoculation time and leaf position
Therefore, the division of races in various regions is not only arbitrarily large, but also it is difficult to compare the results with other regions in China and abroad
Unscientific vaccination methods may also lead to lack of accuracy and reliability of results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] 1. The 24 rice varieties with known disease resistance genes are from the China Rice Research Institute, which is a set of near-isogenic line materials bred by the International Rice Research Institute (IRRI). See Table 2. Transplant the tested rice varieties to each demarcated plot in the specified order, plant one row for each variety, plant a single plant, and the row spacing is 25×20cm, soak seedlings in mid-April, and transplant in mid-May .

[0020] rice variety

resistance gene

rice variety

resistance gene

IRBB1

IRBB2

IRBB3

IRBB4

IRBB5

IRBB7

IRBB8

IRBB10

IRBB11

IRBB13

IRBB14

IRBB21

Xa1

Xa2

Xa3

Xa4

xa5

Xa7

Xa8

Xa10

Xa11

xa13

Xa14

Xa21

IR24

IRBB50

IRBB51

IRBB52

IRBB53

IRBB54

IRBB55

IRBB56

IRBB57

IRBB58

IRBB59

IRBB60

reincarnated parent

Xa4+xa5

Xa4+xa13

...

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PUM

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Abstract

A method for dividing the bacterial blight strain of rice into microspecies by using the 6 subisopolar gene lines of known disease-resistant genes is disclosed. In the booting stage of rice, the bacterial suspension is inoculated to the leaf on the master stem of rice. The longth of disease spot and the length of whole leaf are respectively measured in 2 weeks and 3 weeks. If the length of disease spot is less than 0.25 times that of whole leaf, it is a disease-resistant plant (R). Otherwise, it is a disease-infectious plant(s). 6 varieties of rice are experimented in the interaction mode of 9 micropecies (R1-R9) to obtain the relative microspecies of bacterial blight strain.

Description

(1) Technical field [0001] The invention is a method for classifying bacterial blight of rice, which is specially used for dividing and identifying the races of bacterial blight of rice, so as to timely and accurately grasp the race composition and distribution of dominant races of bacterial blight of rice, so as to facilitate rice Bacteria blight resistance breeding and the rational distribution of field varieties are also conducive to the comparison and research of different races. (2) Background technology [0002] Rice bacterial blight is one of the most important bacterial diseases of rice production in Asia and the Pacific. The breeding and utilization of disease-resistant varieties is the most economical and effective measure to control the disease, but the identification of disease-resistant genes, the breeding and screening of disease-resistant varieties, and the rational distribution of varieties all depend on the research on the differentiation of pathogen races. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/22
Inventor 刘凤权胡白石杨万风刘红霞
Owner NANJING AGRICULTURAL UNIVERSITY
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