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Plant cells and plants which synthesize a starch with an increased final viscosity

A plant cell and plant technology, applied in the field of plant cells and plants, can solve the problems of high final viscosity and unobtained

Active Publication Date: 2006-01-25
BAYER INTELLECTUAL PROPERTY GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Higher final viscosities have not been obtained so far in RVA analysis

Method used

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  • Plant cells and plants which synthesize a starch with an increased final viscosity
  • Plant cells and plants which synthesize a starch with an increased final viscosity
  • Plant cells and plants which synthesize a starch with an increased final viscosity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0288] Analysis of starch from plants with reduced expression of BEI, SSIII and BEII genes

[0289] Starch from different independent lines of transformants 108CF and 110CF described in Example 1 was isolated from potato tubers. The physicochemical properties of this starch were then analyzed. Characterization results of the modified starches are shown in Table 2 (Tab. 2) as examples for some selected plant lines. Analysis was performed by the method described above.

[0290] Tables 2, 3 and 4 below summarize the results of the RVA analysis based on starch from wild-type plants:

[0291] RVA

[0292] Table 2: from wild-type plants (cv.Desiree), plants with reduced SSIII and BEI protein activity (038VL008, 038VL107), and plants with reduced SSIII, BEI and BEII protein activity (108CF041, 110CF003) RVA analysis parameters for starch expressed as a percentage based on wild-type starch data. RVA analysis was performed as described in Assay 1.

[0293] R...

Embodiment 3

[0320] Analysis of Side Chain Distribution of Amylopectin by Gel Permeation Chromatography

[0321] For the separation of amylose and amylopectin, 100 mg of starch were dissolved in 6 ml of 90% strength (v / v) DMSO under constant stirring. After adding 3 volumes of ethanol, the precipitate was separated by centrifugation at 1800 g for 10 minutes at room temperature. The precipitate was then washed with 30 ml of ethanol, dried and dissolved in 10 ml of 1% strength (w / v) NaCl solution at 60°C. After the solution was cooled to 30°C, about 50 mg of thymol was slowly added, and the solution was incubated at 30°C for 2-3 days. The solution was then centrifuged at 2000g for 30 minutes at room temperature. The supernatant was treated with 3 volumes of ethanol, and the precipitated amylopectin was separated by centrifugation at 2000 g for 5 minutes at room temperature. The pellet (pullulan) was washed with 10 ml of 70% strength (v / v) ethanol, centrifuged at 2000 g for 10 minutes at r...

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Abstract

The present invention relates to a plant cell which is genetically modified, the genetic modification leading to the reduction of the activity of one or more SSIII proteins which occur endogenously in the plant cell and to the reduction of the activity of one or more BEI proteins which occur endogenously in the plant cell and to the reduction of the activity of one or more BEII proteins which occur endogenously in the plant cell in comparison with corresponding plant cells, of wild-type plants, which have not been genetically modified. Further aspects of the invention relate to plants containing such plant cells, to a method for generating the plant cells and plants, and to the starch obtainable from them.

Description

technical field [0001] The present invention relates to genetically modified plant cells and plants which result in a reduced activity of the SSIII protein and the BEI and BEII proteins compared to corresponding plant cells of wild-type plants which have not been genetically modified. Furthermore, the present invention relates to means and methods for producing such plant cells and plants. Said plant cells and plants synthesize modified starch characterized by: its amylose content of at least 30%; increased; in RVA analysis its final viscosity is higher than prior art starches; and / or has altered side chain distribution; and / or has increased gel strength in texture analyzers; and / or has Altered particle morphology and / or altered average particle size. The present invention therefore also relates to the plant cells and plant-synthesized starches according to the invention, as well as methods for producing such starches. Background technique [0002] In view of the present ...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N15/82C12N5/10A01H5/00A01H5/10A23L1/0522C12N9/10
CPCA23V2002/00C12N15/8245C12N9/107C12N9/1051A23L1/0522A23L29/212A23V2300/21
Inventor M·赫内C·弗罗贝格V·兰德许茨
Owner BAYER INTELLECTUAL PROPERTY GMBH
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