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Method for directly taking root of tea-tree tissue culture seedling

A technology for tissue culture seedlings and tea trees, applied in the field of seedling raising, can solve the problems of waste of resources, the survival rate is less than 50%, the application limitation of tissue culture technology, etc., and achieves improved survival and rooting rate, good growth, and improved transplanted rooting survival rate. Effect

Inactive Publication Date: 2006-03-08
TEA RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the rooting rate of rooting method in test tube generally only has about 60% at present, and the cycle of rooting is about 60 days, because the root that the tissue culture seedling forms in test tube is all young and tender fleshy root, after being transplanted in the substrate, very Most of them died due to the inadaptability of the roots to the substrate, resulting in a transplanted survival rate of less than 50%, resulting in a huge waste of resources and limiting the application of tissue culture techniques in tea seedling cultivation. needs, and the problem of cultivating improved varieties of tea gardens needs to be resolved urgently
The solution to the problem of improved species is the rapid propagation of new species of seedlings through tissue culture, and rooting technology has become a bottleneck in the application of tissue culture technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Taking the rooting of Longjing 43 tissue culture seedlings as an example, the specific description of the direct rooting method is as follows:

[0013] 1. Obtaining of Tea Tree Tissue Culture Transplanted Seedlings

[0014] (1) The test material is tea tree tissue culture seedlings, and the source of explants is axillary buds. Select the tissue-cultured seedlings with a height of about 5-6cm and relatively consistent growth conditions, transfer them to MS medium for about 10 days, open the bottle 3 days before the test, and put them in the greenhouse for 3 days to harden;

[0015] (2) Take out the tissue culture seedling from the culture bottle, wash the culture medium at the bottom, remove the brown callus produced at the base, and rinse with carbendazim solution.

[0016] 2. Rooting treatment of tea tree tissue culture seedlings

[0017] A certain concentration of hormone is used to treat the base of the tea tree tissue culture seedlings, and then the cuttings are i...

Embodiment 2

[0025] Taking the rooting of Longjing long-leaf tissue culture seedlings as an example, the specific description of the direct rooting method is as follows:

[0026] (1) Obtaining of Tea Tree Tissue Culture Transplanting Seedlings

[0027] 1. The test material is tea tree tissue culture seedlings, and the source of explants is axillary buds. Select tissue-cultured seedlings with a height of about 5-6 cm and relatively consistent growth conditions, transfer them to MS medium for about 10 days, open the bottle 3 days before the test, and put them in the greenhouse for 3 days to harden the seedlings.

[0028] 2. Take out the tissue culture seedlings from the culture bottle, wash the medium at the bottom, remove the brown callus produced at the base, and rinse with carbendazim solution.

[0029] (2) rooting treatment of tea tree tissue culture seedlings

[0030] Treat the base of the tea tree tissue culture seedlings with a certain concentration of hormone, and then insert the c...

Embodiment 3

[0038] Taking the rooting of China Tea 102 tissue culture seedlings as an example, the specific description of the direct rooting method is as follows:

[0039] (1) Obtaining of Tea Tree Tissue Culture Transplanting Seedlings

[0040] 1. The test material is tea tree tissue culture seedlings, and the source of explants is axillary buds. Select tissue-cultured seedlings with a height of about 5-6 cm and relatively consistent growth conditions, transfer them to MS medium for about 10 days, open the bottle 3 days before the test, and put them in the greenhouse for 3 days to harden the seedlings.

[0041] 2. Take out the tissue culture seedlings from the culture bottle, wash the medium at the bottom, remove the brown callus produced at the base, and rinse with carbendazim solution.

[0042] (2) rooting treatment of tea tree tissue culture seedlings

[0043] Treat the base of the tea tree tissue culture seedlings with a certain concentration of hormone, and then insert the cuttin...

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Abstract

The present invention discloses a tea tree tissue-cultured seedling directly-rooting method capable of raising survival rate of tea tree tissue-cultured seedlings, simplifying rooting operation program and shortening culture period. Said method includes the following steps: using tea tree tissue-cultured seedling as experimental material, utilizing a hormone with a certain concentration to make treatment, after 60 days, the survival rate of tea tree tissue-cultured seedlings can be up to above 70%, the rooting rate of survival seedling is up to 90%, and the average root number and root length are respectively 9.4 and 3.64cm.

Description

technical field [0001] The invention belongs to a seedling raising method, and mainly relates to a method for direct rooting of tea tree tissue cultured seedlings, in particular to a rapid and efficient rooting method for tissue cultured seedlings induced by hormones in a greenhouse. Background technique [0002] The propagation coefficient of short spike cuttings of tea trees is about 1:50-60, which is far from that of field crops (above 1:1000). Due to the low reproduction coefficient, after a new tea tree variety is bred, it usually takes about 10 years for the original species to multiply before reaching a certain scale of seedling raising capacity, while the variety advantages of the new variety will take longer to be reflected in production. Therefore the cultivation and popularization speed of tea tree new variety are very slow. Utilize tissue culture technology to breed seedlings rapidly, improve reproduction coefficient, but tissue culture technology has not been r...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00A01H4/00A01H3/04
Inventor 成浩周健曾建明王丽鸳
Owner TEA RES INST CHINESE ACAD OF AGRI SCI
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