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Method for tissue culture and rapid propagation of cortex acanthopanacis

A technique of tissue culture rapid propagation and Acanthopanax bark, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low germination rate of Acanthopanax bark seeds and inability to meet large-scale production

Inactive Publication Date: 2016-12-07
李军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the low germination rate of Acanthopanax bark seeds, it is far from meeting the needs of large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Bud induction culture: Take the budd stem segment of Acanthopanax senticosus tree as explants, cut off the leaves, and cut into 2cm-long segments with 1 axillary bud, soak in detergent solution for 4min, and then use soft hair Brush gently on the surface of the explants to remove dust and part of the bacteria on the surface, then rinse with tap water for 3 hours and place in an ultra-clean workbench, disinfect with 75% ethanol for 4 seconds, wash with sterile water 4 times, and then use 0.1 Disinfected with% mercury solution for 15 minutes, rinse with sterile water 5 times and wipe off the water droplets on the surface with sterile filter paper, and then inoculate the bud induction medium for bud induction. After inoculation, cultivate in the dark at 26℃ for 2 days. Then it was cultivated for 28 days under the condition of 9 hours of light every day and light intensity of 1300lx. The bud induction rate and growth situation were counted. The bud induction rate was 88%....

Embodiment 2

[0020] (1) Bud induction culture: take the budd stem segment of Acanthopanax senticosus tree as explants, cut off the leaves, and cut into 6cm long segments with 5 axillary buds, soak in detergent solution for 12 minutes, and then use soft hair Brush gently on the surface of the explant to remove the dust and part of the bacteria on the surface, then rinse with tap water for 2 hours, then place it in an ultra-clean workbench, disinfect with 75% ethanol for 18 seconds, wash with sterile water 8 times, and then use 0.1 Disinfected with% mercury solution for 20 minutes, rinse with sterile water 5 times and wipe off the surface water droplets with sterile filter paper, then inoculate the bud induction medium for bud induction. After inoculation, cultivate in the dark at 26℃ for 4 days. Then irradiate for 12 hours every day and culture for 33 days under the condition of 2500lx light intensity. Statistics of its bud induction rate and growth situation show that the bud induction rate ...

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Abstract

The invention discloses a method for tissue culture and rapid propagation of cortex acanthopanacis, namely a method for obtaining a large number of good cortex acanthopanacis nursery stocks within a short period of time by means of the plant tissue culture technique. A cortex acanthopanacis stem with buds is used as explant, in-vitro regeneration of cortex acanthopanacis is achieved through shoot induction, shoot enrichment culture, rooting culture, seedling hardening, transplantation and the like, and technical support is provided for mass breeding, rapid propagation and new species popularization. The method has the advantages that propagation and growth are fast, root number is large, roots are dense, seedlings are robust, drug property is strong and efficacy is excellent.

Description

Technical field [0001] The invention relates to the field of acanthopanax bark tree planting, in particular to a method for tissue culture and rapid propagation of acanthopanax bark. Background technique [0002] Acanthopanax senticosus is a plant of the Araliaceae family, the dried root bark of Acanthopanaxgracilistylus W.W.Smith. Mainly produced in Hubei, Henan, Sichuan, Hunan and Anhui. Dig the roots in summer and autumn, peel them off with a knife while they are fresh or crack the roots, wash and dry. Medicinal properties: The root bark is in an irregular roll shape, with a length of 5 to 15 cm, a diameter of 0.4 to 1.4 cm, and a thickness of about 0.2 cm. The outer surface is grayish brown, with slightly twisted longitudinal wrinkles and horizontally long lenticels, and the inner surface is pale yellow or grayish yellow with fine longitudinal lines. Light body, brittle, easy to break, irregular section, off-white. Slightly fragrant, slightly spicy and bitter taste. The ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 李军
Owner 李军
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