Application of amino acid transport gene OsAAP5 to rice breeding

A technology of amino acid and rice, which is applied in application, genetic engineering, plant genetic improvement, etc., can solve the problems of growth and development without any research

Active Publication Date: 2017-04-26
WUHAN INST OF BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the research on rice AAP family members, the rice amino acid transpo

Method used

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  • Application of amino acid transport gene OsAAP5 to rice breeding
  • Application of amino acid transport gene OsAAP5 to rice breeding
  • Application of amino acid transport gene OsAAP5 to rice breeding

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Example 1 OsAAP5 Gene interference plant construction

[0022] The RNA of Hua 11 in rice was extracted and reverse transcribed into cDNA, using primer pairs:

[0023] F1: 5'- GGTACC GCGGAGAACAAGACGATGAA-3'( Kpn I),

[0024] R1: 5'- GGATCC ATGGGCTGGCAGAACACC-3'( Bam H I);

[0025] F2: 5'- ACTAGT GCGGAGAACAAGACGATGAA-3'( Spe I),

[0026] R2: 5'- GAGCTC ATGGGCTGGCAGAACACC-3'( Sac I);

[0027] PCR amplified OsAAP5 The cDNA fragment of the gene was digested with the above-mentioned corresponding restriction enzymes and then connected to the pTCK303 vector to construct OsAAP5 Gene interference expression vector OsAAP5 -pTCK303. Using Agrobacterium EHA105-mediated genetic transformation method, the interference expression vector was introduced into normal japonica rice variety Zhonghua 11.

[0028] Transplant all the transgenic seedlings obtained in a basket with soil, water and fertilize regularly, and when the seedlings grow to about 10cm in height, soak 50 transgenic rice seed...

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Abstract

The invention discloses an application of an amino acid transport gene OsAAP5 to rice breeding, and belongs to the field of plant gene engineering. An amino acid sequence of a protein encoded by the gene OsAAP5 is shown in SEQ ID NO.1, and a cDNA sequence is shown in SEQ ID NO.2. By constructing the rice gene OsAAP5 for plant interference, it is discovered that the root length, root number, plant height and fresh weight of normal rice can be increased by reducing expression of the gene OsAAP5, so that the gene OsAAP5 can be used in the rice breeding to facilitate rice growth and increase rice biomass. The gene OsAAP5 has an important application value in the aspect of describing the influence of amino acid transport on the plant growth and development process.

Description

Technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to amino acid transport genes OsAAP5 Application in rice breeding. Background technique [0002] Plants obtain nitrogen by absorbing ammonia, nitrate, amino acids, soluble peptides, etc. in the soil; the absorption and transport of nitrogen mainly rely on ammonium transport protein (AMT), nitrate transport protein (NRT), and amino acid transport protein (AAT) , Peptide transport protein (PTR) and other transport proteins (Williams L, Miller A. Transporters responsible for the uptake and partitioning of nitrogenous solutes. Annu Rev Plant Biol and Plant Mol Biol, 2001, 52: 659-688.). After ammonium is absorbed by plant AMT, glutamine synthetase (GS) and glutamate synthase (GOGAT) are used to synthesize glutamine and glutamate, and the latter further form other amino acids (SonodaY, Ikeda A, Saiki S, et al. Feedback regulation of the ammonium transportergene family AMT1...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N9/14C12N15/55A01H5/00
CPCC12N9/14C12N15/8205C12N15/8261C12Y306/03
Inventor 方中明吕凯吴博文魏倩钱焌杰寇家豪
Owner WUHAN INST OF BIOENG
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