Kit for ribonuclease protecting experiment

A technology of ribonuclease and kit, which is applied in the measurement/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of no convenient and economical kits, expensive imported reagents, and increased difficulty of observation, etc. Effects of hybridization time, hybridization efficiency improvement, and volume increase

Inactive Publication Date: 2006-06-28
PEKING UNIV SHENZHEN HOSPITAL
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Problems solved by technology

However, using existing kits requires a long hybridization time, and during the experiment of digesting unhybridized single-stranded RNA, the precipitation of undigested protection fragments is not sufficient, which increases the difficulty of observation
[0005] In addition, there are very few reports on the use of this method in China. One of the important reasons is that there is currently no conve

Method used

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  • Kit for ribonuclease protecting experiment
  • Kit for ribonuclease protecting experiment

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Embodiment Construction

[0019] Below in conjunction with the best embodiment shown in the accompanying drawings, it will be further described in detail.

[0020] Such as figure 1 As shown, the ribonuclease protection experiment kit of the present invention includes a box body 1 and a plurality of reagent containers placed in the box body 1, and the plurality of reagent containers are respectively fixed through the positioning holes 21 on the connector 2 Inside the box body 1 , the outer surface of the box body 1 is printed with suggestive information related to the ribonuclease protection experiment. The connecting piece 2 for fixing the reagent container is a horizontal shelf plate with supporting legs and matched with the inner cavity of the box. The positioning holes 21 are arranged on the horizontal shelf plate 2 at a certain distance, and there are 13 in total, arranged in three rows, wherein the diameters of the four positioning holes at the four corners are relatively large, and each reagent ...

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Abstract

The invention relates to a ribonuclease protecting experiment kit. It includes kit body and many reagent containers. The many reagent containers are fixed in the kit body. The kit body includes hybridization, RNA enzyme digestion, probe dilution, and sample adding buffer solution containers. The hybridization bugger solution is the following mixture: 40mmol/L PIPES(pH6.8), 1mmol/L EDTA(pH8.0), 0.4mol/L NaCl, 1mmol/L CTAB, and deionization formamide with 80% volume ratio. The RNA enzyme digestion bugger solution is the following mixture: 0.3mol/L NaCl, 5mmol/LEDTA, 10mmol/L Tris-HCl(pH7.4) and 50mg/L glycogen. The invention has the advantages of high sensitivity, good repeatability, and low production cost. And it can be applied to non radioactivity biotin sign probe.

Description

technical field [0001] The invention relates to reagents used in gene detection, in particular to kits used in messenger ribonucleic acid (mRNA) analysis. Background technique [0002] The expression of a gene needs to go through transcription (mRNA production), translation (protein synthesis), modification and other processes, and finally form a protein molecule with biological activity. mRNA detection is the most important and commonly used method to study gene expression and function. Currently commonly used mRNA detection methods include Northern blot, RT-PCR and ribonuclease protection assay (Ribonuclease protection assay, RPA). [0003] The Northern blot method is one of the classic methods for mRNA detection, which can semi-quantitatively analyze the level of mRNA, but the method is complicated to operate, takes a long time, requires high integrity of the specimen, requires a large amount of samples, and cannot be detected at the same time Multiple target genes; Qua...

Claims

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Application Information

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IPC IPC(8): C12Q1/25C12Q1/68
Inventor 桂耀庭蔡志明刘春霖
Owner PEKING UNIV SHENZHEN HOSPITAL
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