Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Competitive enzyme-linked immune detection method of hepatitis E virus neutralization antibody

A hepatitis E virus, enzyme-linked immunoassay detection technology, applied to measuring devices, material analysis through observation of the impact on chemical indicators, instruments, etc., can solve the problems of cumbersome, expensive, and time-consuming methods, and achieve good safety , low cost, and low production and operation costs

Inactive Publication Date: 2006-06-28
SOUTHEAST UNIV
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The objective of the invention is: develop hepatitis E virus neutralizing antibody competitive ELISA method, replace hepatitis E virus in vivo and (or) in vitro neutralization test, overcome loaded down with trivial details and expensive in vivo and (or) neutralization test method , time-consuming and labor-intensive shortcomings, to evaluate the effectiveness of vaccines

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Competitive enzyme-linked immune detection method of hepatitis E virus neutralization antibody
  • Competitive enzyme-linked immune detection method of hepatitis E virus neutralization antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] The following examples illustrate the invention in detail:

[0010] 1. Acquisition of coated antigen

[0011] Coating antigens come from recombinant proteins or polypeptides encoded by the open reading frame 2 of hepatitis E virus of different genotypes and subtypes or fragments thereof, and can form coating antigens alone or in combination. The acquisition of the coated antigen will be described in detail below by taking seven representative strains of hepatitis E virus as examples.

[0012] The 7 strains of HEV are the Burmese strain of HEV from genotype Ia subtype (M73218), the Pakistani strain of HEV from subtype Ib (M80581), and the Moroccan strain of HEV from subtype Ic. strain (AY230202), the Mexican strain of HEV from genotype II (M74506), the American strain of HEV from genotype III (AF060668) and the New Zealand pig strain of HEV (AF082843), and from the A Chinese strain of hepatitis E virus of genotype IV (AJ272108).

[0013] The nucleotide sequences codin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A competition enzyme ¿C linked immune detection method of neutralization antibody on hepatitis virus in the fifth type includes competing and combining neutralization antibody resisting hepatitis virus in the fifth type with neutralization single clone antibody of enzyme labeled hepatitis virus in the fifth type to encapsulate neutralization antigen tabular position on antigen, realizing competition enzyme ¿C linked immune detection of neutralization antibody on the fifth type of hepatitis virus in biological sample according to color developing decrease degree of enzyme action substrate.

Description

technical field [0001] The invention relates to a detection method of hepatitis E virus neutralizing antibody in serum (serum) belonging to an ELISA detection method of serum (serum) antibody, in particular to a competitive ELISA detection method of hepatitis E virus neutralizing antibody. technical background [0002] Hepatitis E virus (HEV) is a non-enveloped single positive-sense RNA virus that is transmitted through the fecal-oral route, causing acute hepatitis E (Hepatitis E, HE), which usually manifests as acute icteric hepatitis. It develops into subacute severe hepatitis or cholestatic hepatitis. According to the statistics of 27 kinds of legally notifiable infectious diseases reported by the Ministry of Health of the People's Republic of China, the incidence of HE is high and the mortality rate is high, showing an increasing trend year by year in recent years. From 2002 to November 2005, the average case fatality rate of HE was about 0.37%, which was far higher tha...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/577G01N21/78G01N33/531G01N33/543
Inventor 孟继鸿戴星梁久红田华赵宇董晨汪源邓蕾张红梅
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com