Strawberry virus elimination method

A virus-free, strawberry technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve problems such as the influence of shoot tip growth, the decline in survival rate, and the inability to achieve detoxification effect.

Inactive Publication Date: 2006-07-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the strawberry returns to the normal growth temperature of about 25°C, the virus will rejuvenate and cannot achieve the detoxification effect
[0007] Stem tip combined with heat treatment method....

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1, a kind of strawberry devirus method, carries out following steps successively:

[0019] 1) Put the potted strawberry seedlings with strawberry mottle virus into a biochemical incubator, and cultivate them at 55-60°C for 2-3 days.

[0020] 2), peeling off the shoot tip meristem of the above-mentioned strawberry seedlings less than 0.5 mm, inoculated into 1 / 2 MS medium to make it grow.

[0021] The 1 / 2 MS medium has half the content of the MS medium, that is, 1 / 2 MS+2-3% sugar+5-9% agar, and the pH is 5.8-6.0.

[0022] 3) When the seedlings produced by the above-mentioned shoot apical meristem grow to a height of 0.5-1.5 cm, transfer to the induction proliferation medium to make it grow; when adventitious buds appear, transfer to a biochemical incubator, and cultivate at 55-60°C for 30 minutes .

[0023] The proliferation-inducing medium is: MS+0.2-0.5 mg / l BA+2-3% sugar+5-9% agar, and the pH is 5.8-6.0.

[0024] 4), and then put the seedlings obtained af...

Embodiment 2

[0027] Embodiment 2, a kind of strawberry devirus method, described virus is strawberry light yellow edge virus, and this method is the same as

[0028] Example 1.

[0029] The obtained strawberry seedlings are tested, and the light yellow edge virus of the strawberry has been effectively removed, and the virus removal rate reaches 100%. And do not need to peel off a large amount of stem tips, generally adopt multi-step step-by-step method as long as peel off 10-20 stem tips.

Embodiment 3

[0030] Embodiment 3, a kind of strawberry devirus method, described virus is following 4 kinds: strawberry mottle virus, strawberry light yellow edge virus, strawberry vein virus, strawberry shrunken virus.

[0031] Change the virus lethal temperature of 55~60°C in step 1) and step 3) to 60~65°C, and the rest are the same

[0032] Example 1.

[0033] The obtained strawberry seedlings have been tested, and the above four kinds of viruses in the strawberry have been effectively removed, and the virus-free rate has reached 100%. And do not need to peel off a large amount of stem tips, generally adopt multi-step step-by-step method as long as peel off 10-20 stem tips.

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PUM

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Abstract

The invention discloses a method for strawberry virus-removing, comprising the following steps: 1) putting the potted strawberry with virus into the bio-chemical incubator, culturing in the lethal temperature for virus for 2-3 days; 2) stripping the stem tip nascent tissue of smaller than 0.5mm, inoculating which in the culture medium with concentration of MS half-reduced for its growing; 3) transferring the stem tip nascent tissue when its sprout grows to 0.5-1.5cm in height to induced enrichment culture medium for growing; transferring it to biochemical incubator when generates adventitious bud, culturing under the lethal temperature for 30 minutes; 4) putting the sprout mentioned above into tissue culture room for culturing under 28 Deg C for 30 minutes; 5) repeating the steps 2) to 4) for 1-2 times. The method in this invention does not strip large quantity of stem tip and the virus-removing rate is as high as 100%.

Description

technical field [0001] The invention relates to a strawberry devirus method. Background technique [0002] Strawberry has been propagated by asexual reproduction for a long time, and stolons are mainly used in production; due to the infection of various pathogens for many years of planting, the original good varieties degenerate and reduce production. According to the production survey, the main virus diseases that harm strawberry are strawberry mottle virus (Strawberry mottlevirus, SMoV), strawberry mild yellow edge virus (Strawberry mild yellow edge virus, SMYEV), strawberry vein banding virus (Strawberry vein banding virus, SVBV) and strawberry Strawberry crinkle virus (SCV), etc. Since the virus invades the plant, it will form a systemic infection and affect the metabolism of the host plant. Therefore, chemical agents are difficult to prevent and control the virus, which makes the virus spread widely. So far, 80% of strawberry production areas in my country are infected...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H3/00C12N5/04A01G7/00A01G1/00
Inventor 毛碧增李德葆
Owner ZHEJIANG UNIV
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