Metarrhizium anisopliae deltoid moths subspecies and its disinsection culture and use method

A technology of silver print moth and metarhizium anisopliae, applied in the field of microorganisms, can solve the problems of narrow insecticidal range and poor control effect

Active Publication Date: 2006-10-04
镇江市润宇生物科技开发有限公司 +1
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although these fungal insecticides have certain insecticidal effects, their insecticidal range is narrow
Especially Noctuidae pests that are more harmful to vegetables have poor control effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0033] The scarab metarhizium anisopliae subspecies of the present embodiment was extracted on December 5, 2004 at the Wuhan China Type Culture Collection Center (CCTCC), and the preservation number is: CCTCCNO: M204078 The Latin scientific name is Metarhizium anisopliae var .argyrogrammaagnata GXW26-8 The same bacterial species, cultivated through the following steps and used to kill Lepidoptera Noctuidae and other pests.

[0034] A. Cultivate the isolated, screened and purified MaGXW26-8 strain with a test tube or a strain bottle at a temperature of 27-28°C, with a light of 3000 Lx, and for 120-130 hours with a pH value of 6.8-6.9.

[0035] B. Select excellent strains with high survival rate on the slope, inoculate them into liquid culture tanks, and carry out liquid culture. The time is preferably controlled within 48 hours ± 2 hours.

[0036] C. Add the strains in the liquid strain tank to the fermenter according to 10% of the culture material in the solid fermenter, and c...

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PUM

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Abstract

The invention relates to a Metarhizium anisopliae var.argyrogramma agnata GXW26-8 (CCTCC No:M204078) for eradicating multiple agronomic crop pests, and also relates to the method for cultivation and use in disinsection. The MaGXW26-8 can be used for developing biological insecticide.

Description

technical field [0001] The invention relates to a fungus, especially a metarhizium anisopliae subspecies that can cause the death of various crop pest morbidity diseases, and the invention also relates to its method for killing insects, cultivating and using it, and belongs to the technical field of microorganisms. Background technique [0002] As far as the applicant knows, Noctuidae pests are pests of most crops, especially vegetable crops. [0003] For a long time, suppressing and eliminating pests has always been the main measure for human beings to increase agricultural income. In recent years, research on biological control of pests has made significant progress. According to the introduction in the book "China's Biological Control Entering the 21st Century" published by China Agricultural Science and Technology Press in April 2005, the fungal biopesticides currently promoted in my country are mainly Beauveria bassiana and Metarhizium anisopliae series products, such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04
Inventor 高小文
Owner 镇江市润宇生物科技开发有限公司
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