Method of separating blood vessel endothelium precursor cell from marrow and cultivating in vitro
A precursor cell, in vitro culture technology, applied in artificial cell constructs, tissue culture, animal cells, etc., can solve problems such as limited number
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[0021] The present invention will be described in detail below with the embodiment of isolating vascular endothelial precursor cells from the bone marrow of Wistar rats and culturing them in vitro:
[0022] A method for isolating vascular endothelial precursor cells from bone marrow and culturing them in vitro, comprising the following steps:
[0023] 1. Harvesting bone marrow cells: Wistar rats were killed by cervical dislocation, soaked in 75% ethanol for about 15 minutes for disinfection. Ultra-clean table, disinfection of surgical instruments. The rat hindlimb was separated from the hip joint, the femur and tibia were exposed, and the blood-stained villi were removed. The scissors cut off the epiphysis at both ends, exposing the bone marrow cavity. Use a 10ml syringe to draw the EBM-2 culture solution containing 100IU / ml heparin, inject it into the bone marrow cavity through a needle, collect the washed bone marrow, blow the harvested bone marrow repeatedly with a syring...
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