36 results about "Endothelial Precursor Cell" patented technology

Described are methods of modulating stem/progenitor cell recruitment involving molecules that agonize the formation of plasmin stimulating the recruitment of stem/progenitor cells, including hematopoietic and endothelial precursor cells. Conversely, antagonists of plasmin can inhibit recruitment of the stem cells. In addition, the identification of the uPA receptor (uPAR) as a retention signal for stem cells in their niche suggests a novel method for increased engraftment and isolation of multipotent stem cells.

In vitro methods are disclosed that rely on a novel phenomenon of secondary sprouting by cultured endothelial precursor cells, after angiogenic-like tube formation and collapse have occurred on a basement membrane matrix. Particularly disclosed is an in vitro method of isolating and expanding, from a mixed population of mammalian cells originating from a tissue sample, a cellular population enriched for endothelial sprout cells, including cells having one or more physiological and/or immunological features of endothelial precursor cells. Also disclosed is an in vitro method for a screening a substance for potential proangiogenic or antiangiogenic activity.

A method for regenerating a blood vessel comprising introducing a cell-containing fluid containing vascular endothelial precursor cells and cells to be removed into a cell separator which allows at least the cells to be removed to substantially pass through but substantially captures the vascular endothelial precursor cells; recovering the vascular endothelial precursor cells once captured on said cell separator by introducing a fluid into the said cell separator; and using the recovered vascular endothelial precursor cells for regenerating a blood vessel.

Disclosed are compositions and methods for promoting or accelerating wound healing and preventing, treating, or reducing symptoms associated with wounds or wounding disorders such as diabetic ulcers and burns. In one embodiment, the method includes administering a therapeutically effective amount of a nucleic acid encoding at least one morphogen, or an effective fragment thereof. Preferred morphogens include the human Sonic Hedghog (Shh), human Desert Hedgehog (Dhh), and human Indian Hedgehog (Ihh) proteins. The methods can be used alone or in combination with other methods involving administration of an angiogenic protein, a hematopoietic protein, or cells such as endothelial cells or endothelial precursor cells.

The invention relates to a corneal posterior lamella. The corneal posterior lamella is prepared by the following method: (a) separating and culturing cat corneal endothelial precursor cells for obtaining corneal endothelial precursor cells; (b) preparing a porcine corneal acellular matrix and obtaining the acellular matrix; and (c) constructing the corneal posterior lamella. The invention further provides a use of using the corneal posterior lamella as a donor for corneal transplantation. The structure of the tissue engineering corneal posterior lamella constructed by the corneal endothelial precursor cells is similar to the structure of the normal corneal posterior lamella. The construction of the corneal posterior lamella by taking the corneal endothelial precursor cells as seeds can provide sufficient cell sources, overcome the difficult problem of low proliferation capacity of the corneal endothelial cells and simultaneously solve the problems of cell transformation and safety.

Compositions and processes for promoting wound healing are provided by the present invention which increase the rate and completeness of wound healing. Broadly described, an inventive composition includes a degradable scaffold of biocompatible material and a cell involved in wound healing, or a predecessor of such a cell, disposed in contact with the scaffold. In one embodiment, an albumin scaffold is provided with an endothelial cell predecessor disposed therein. Optionally, a cell involved in wound healing or a predecessor of such a cell is administered by local or systemic injection in conjunction with application of an inventive composition. Processes for promoting wound healing are provided as well as processes for producing the described compositions for promoting healing.

The present invention provides methods for producing hematopoietic stem cells or vascular endothelial precursor cells, wherein the methods comprise the step of separating PCLP1-positive cells from the hematopoietic tissues of an individual, and then culturing the obtained cells. PCLP1-positive cells obtained from the hematopoietic tissues of an individual can be cultured for a long time, and during culture they produce large quantities of hematopoietic stem cells or vascular endothelial precursor cells. The hematopoietic stem cells or vascular endothelial precursor cells obtainable by the present invention can be utilized for regenerative medicine.

AC133⁺/CD34⁺ cells isolated from human bone marrow can be stimulated with the pro-angiogenic factors VEGF, bFGF, and heparin, resulting in the generation of a population of cells that is adherent and possesses many of the same properties as mature endothelial cell types, HMVECs and HUVECs. The newly-formed, endothelial-like cells are referred as adherent endothelial precursor cells (aEPCs); these cells appear to be intermediates between haematopoietic stem cells (HSCs) and mature endothelial cells. Direct comparison of aEPCs with HMVECs and HUVECs in several in vitro functional assays, such as tube formation, migration, invasion, and expression of cells surface markers, reveals differences and similarities. In a Matrigel™ matrix angiogenesis assay the aEPCs form vessels in vivo and interact with human ovarian cancer cells. Mouse cell lines that are useful models for tumor endothelial cells are identified by determining mRNA and protein expression levels of murine homologs of tumor endothelial markers. Mouse cell lines selected as models for tumor endothelial cells can be used to evaluate pro-angiogenic and anti-angiogenic factors.

The invention relates to the use of erythropoietin for stimulation of the physiological mobilisation, proliferation and differentiation of endothelial precursor cells, for stimulation of vasculogenesis, for the treatment of diseases related to a dysfunction of endothelial precursor cells and for the production of pharmaceutical compositions for the treatment of such diseases and pharmaceutical compositions comprising erythropoietin and other agents suitable for stimulation of endothelial precursor cells.

The invention provides a use of CD133<+> endothelial precursor cells, and concretely relates to a use of the CD133<+> endothelial precursor cells in the preparation of medicinal compositions for treating diabetic foot. The specific endothelial precursor cells can substantially improve the symptoms of diabetic foot patients in a specific intra-arterial application mode, and even reverse serious diabetic foot patients in order to provide a new clinic application for the treatment of the diabetic foot. The CD133<+> endothelial precursor cells are autologous, are used in autograft, have no ethical defects, and are safe and feasible.

A treatment method capable of regenerating new blood vessels at an affected site of a peripheral blood vessel that is highly safe with respect to the human body and is easily performed. More specifically, a vascularization therapy that increases the number of newly formed blood vessels of an affected site by immersing in carbonated warm water having a carbon dioxide concentration of 700 ppm or more and a water temperature of 33 to 42° C. A vascularization therapy that increases the number of vascular endothelial cells in tissue of an affected site by 1.5 times or more and that increases the number of endothelial precursor cells in the peripheral blood at an affected site by 1.1 times or more by immersing the affected site of a peripheral blood vessel in the aforementioned carbonated warm water.

The invention provides preserving fluid for long-term preservation of endothelial precursor cell for over-expressing VEGF. The preserving fluid comprises the following components: albumin, glucose, glycol, dimethyl sulfoxide, epidermal growth factor, magnesium sulfate, L-glutamine and betamethasone. The invention further provides an application method of the preserving fluid. Through the application method, the endothelial precursor cell for over-expressing VEGF is preserved with the preserving fluid, the endothelial precursor cell for over-expressing VEGF can be preserved for a long time, andresuscitated cell can properly maintain a proliferation function and biological characteristics of the endothelial precursor cell.

The invention discloses the method for separating vessel esoderma fore-body cell from bone marrow and extracorporal culturing. The method comprises the following steps: 1 getting marrow cell; 2 collecting individual cell; 3 making EBM-2 cell culture liquid; 4 cell culturing; 5 passage culturing. The bFGF and VEGF are added in EBM-2 cell culture liquid to improve proliferation of vessel esoderma fore-body cell. So the invention can get many vessel esoderma fore-body cells in a short time.

The invention relates to the use of low-dose erythropoietin for stimulating physical mobilization, proliferation, and differentiation of endothelial precursor cells, stimulating vasculogenesis, treating diseases that are linked to a dysfunction of endothelial precursor cells, and producing pharmaceutical compositions used for the treatment of such diseases as well as pharmaceutical compositions containing erythropoietin and other appropriate agents for stimulating endothelial precursor cells.