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Non-human mammal embryonic tissue cell suitable for cell transplantation, its preparation method and application

A technology of non-human mammals and tissue cells, applied in the direction of mammalian medical raw materials, biochemical equipment and methods, embryonic cells, etc., can solve problems such as time-consuming, troublesome immune rejection, complex equipment, etc.

Inactive Publication Date: 2007-05-23
张晓凤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the measures for reducing the immune rejection in cell transplantation therapy in the prior art are all cumbersome, requiring complex equipment and taking a long time to implement.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1 Preparation of sheep embryo testis germinal stromal cells

[0046] 1. Obtaining Sheep Fetal Tissue

[0047] Take the ewe sheep that have been pregnant for 4-8 weeks and the fetus is male by B-ultrasound examination, and 10ml~15ml~20ml of blood from male subjects who are going to receive embryonic cell activation therapy (finally determined according to the age of the person receiving treatment) 10 ml of blood from a 40-50-year-old man, 15 ml of blood from a 50-60-year-old man, and 20 ml of blood from a 60-70-year-old man), intravenously injected into the sheep. After maintaining the stimulation for 2-6 weeks, the ewes were anesthetized, and the sheep fetuses were removed by dissection.

[0048] Take out the bilateral testes through small incisions for local routine disinfection, rinse them with normal saline, put them into a sterilized jar filled with 70% alcohol, and put the specimen bottle into an ice shell with a temperature of 0°C-4 ℃, sent to the lab...

Embodiment 2

[0061] Example 2 Preparation of sheep embryonic ovary germinal stromal cells

[0062] 1. Obtain the tissue of sheep embryo. Carried out in a manner similar to Example 1, but using blood from a female subject to inject a pregnant ewe with a female fetus (final determination is made according to the age of the person receiving treatment, blood from a 30-40-year-old woman is injected with 10 ml , inject 15ml of blood from women aged 40 to 50, inject 20ml of blood from women aged 50 to 60), and take fetal ovaries to prepare embryonic cells.

[0063] 2, germinal stromal cell culture (same as embodiment 1)

[0064] 3. Confirm the state of the reproductive stromal cells (same as Example 1)

[0065] 4. Trial on human subjects (same as Example 1)

[0066] Ovarian cells were collected and confirmed by cell counting that the number reached 2×10 6 ~2×10 7 ~2×10 8 . Inject it into the muscle of the subject within a volume of 5ml-10ml to complete the transplantation of female ovarian...

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Abstract

The invention relates to non-human mammal embryonic tissue cells for cellular transplant, its preparation method and application.

Description

technical field [0001] The present invention relates to non-human mammalian embryonic cells suitable for cell transplantation to improve the health status of individuals, their preparation methods and uses. Background technique [0002] Since ancient times, human beings have always expected to have good methods for improving health, delaying aging, and maintaining youth forever. Therefore, many attempts have been made and great progress has been made. Organ transplantation is one such effective treatment. [0003] Currently, organ transplant therapy has been successfully used to treat a variety of diseases. However, because many organ diseases are not suitable for treatment with whole organ transplantation, for example, damage to the central nervous system cannot be replaced by whole organ transplantation. Cell transplantation therapy was developed on this basis. [0004] Cell transplantation therapy is a method of injecting, transplanting or placing cells in other ways i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/06A61K35/12A61K35/48C12N5/073
Inventor 张晓凤
Owner 张晓凤