Water-soluble rhodamine dye conjugates

a technology of rhodamine dye and conjugate, which is applied in the field of fluorescent dye compounds, can solve the problems of adversely affecting the synthesis of rhodamine dyes

Inactive Publication Date: 2003-03-20
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Problems solved by technology

However, electron-withdrawing groups (e.g., --NO.sub.2, --F, --Cl, --CN, --CF.sub.3, etc.) should not be attached directly to the pyridinium ring carbons, as these substituents may adversely affect the synthesis of the rhodamine dyes.

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[0027] 5.1 Abbreviations

[0028] The abbreviations used throughout the specification to refer to certain nucleobases, nucleosides and / or nucleotides are those commonly employed in the art and are as indicated below:

1 Expression Abbreviation adenine A 7-deazaadenine 7-deaza-A N.sup.6-.DELTA..sup.2-isopentenyladenine 6iA N.sup.6-.DELTA..sup.2-isopentenyl-2-methylthioadenine 2ms6iA cytosine C guanine G 6-thioguanine 6sG 7-deazaguanine 7-deaza-G N.sup.2-dimethylguanine 2dmG 7-methylguanine 7mG thymine T 4-thiothymine 4sT uracil U dihydrouracil D 4-thiouracil 4sU base Y Y ribonucleoside-5'-triphosphate NTP adenosine-5'-triphosphate ATP 7-deazaadenosine-5'-triphosphate 7-deaza-ATP cytidine-5'-triphosphate CTP guanosine-5'-triphosphate GTP 7-deazaguanosine-5'-triphosphate 7-deaza-GTP thymidine-5'-triphosphate TTP uridine-5'-triphosphate UTP 2'-deoxyribonucleoside-5'-triphosphate dNTP 2'-deoxyadenosine-5'-triphosphate dATP 2'-deoxy-7-deazaadenosine-5-'-triphosphate 7-deaza-dATP 2'-deoxycytidi...

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Abstract

The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and / or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.

Description

[0001] This application is a division of pending application Ser. No. 09 / 661,206, filed on Sep. 14, 2000, which is a division of application Ser. No. 09 / 433,093, filed on Nov. 3, 1999, now U.S. Pat. No. 6,191,278, issued Feb. 20, 2001, all of which are incorporated herein by reference.1. FIELD OF THE INVENTION[0002] The present invention relates generally to fluorescent dye compounds that are useful as molecular probes. In particular, the present invention relates to fluorescent rhodamine dye compounds that are photostable and highly water-soluble.2. BACKGROUND OF THE INVENTION[0003] The non-radioactive detection of nucleic acids utilizing fluorescent labels is an important technology in modem molecular biology. By eliminating the need for radioactive labels, safety is enhanced and the environmental impact and costs associated with reagent disposal is greatly reduced. Examples of methods utilizing such non-radioactive fluorescent detection include automated DNA sequencing, oligonucl...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07D311/80C07D491/04G01N33/53C07D491/06C07H19/207C07H21/00C07K1/13C09B11/24C09B11/28C09K11/06C12N15/09C12Q1/68G01N33/533G01N33/543G01N33/58
CPCC07D311/80C07D491/04C07D491/06C07H19/207C07H21/00C09B11/24G01N33/582G01N2458/00Y10S436/80
Inventor LEE, LINDA G.GRAHAM, RONALD J.WERNER, WILLIAM E.SWARTZMAN, ELANALU, LILY
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