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Secreted and transmembrane polypeptides and nucleic acids encoding the same

a technology of applied in the field of secreted and transmembrane polypeptides and nucleic acids encoding the same, can solve the problems of loss of biological activity and possible changes in immunogenicity

Inactive Publication Date: 2003-09-25
GENENTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, it is also recognized that, in some cases, cleavage of a signal sequence from a secreted polypeptide is not entirely uniform, resulting in more than one secreted species.
When encapsulated antibodies remain in the body for a long time, they may denature or aggregate as a result of exposure to moisture at 37.degree. C., resulting in a loss of biological activity and possible changes in immunogenicity.

Method used

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  • Secreted and transmembrane polypeptides and nucleic acids encoding the same
  • Secreted and transmembrane polypeptides and nucleic acids encoding the same
  • Secreted and transmembrane polypeptides and nucleic acids encoding the same

Examples

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example 2

[0425] Isolation of cDNA Clones by Amylase Screening

[0426] 7. Preparation of Oligo dT Primed cDNA Library

[0427] mRNA was isolated from a human tissue of interest using reagents and protocols from Invitrogen, San Diego, Calif. (Fast Track 2). This RNA was used to generate an oligo dT primed cDNA library in the vector pRK5D using reagents and protocols from Life Technologies, Gaithersburg, Md. (Super Script Plasmid System). In this procedure, the double stranded cDNA was sized to greater than 1000 bp and the Sall / NotI linkered cDNA was cloned into XhoI / NotI cleaved vector. pRK5D is a cloning vector that has an sp6 transcription initiation site followed by an Sfil restriction enzyme site preceding the XhoI / NotI cDNA cloning sites.

[0428] 2. Preparation of Random Primed cDNA Library

[0429] A secondary cDNA library was generated in order to preferentially represent the 5' ends of the primary cDNA clones. Sp6 RNA was generated from the primary library (described above), and this RNA was use...

example 3

[0449] Example 3

[0450] Isolation of cDNA Clones Using Signal Algorithm Analysis

[0451] Various polypeptide-encoding nucleic acid sequences were identified by applying a proprietary signal sequence finding algorithm developed by Genentech, Inc. (South San Francisco, Calif.) upon ESTs as well as clustered and assembled EST fragments from public (e.g., GenBank) and / or private (LIFESEQ.RTM., Incyte Pharmaceuticals, Inc., Palo Alto, Calif.) databases. The signal sequence algorithm computes a secretion signal score based on the character of the DNA nucleotides surrounding the first and optionally the second methionine codon(s) (ATG) at the 5'-end of the sequence or sequence fragment under consideration. The nucleotides following the first ATG must code for at least 35 unambiguous amino acids without any stop codons. If the first ATG has the required amino acids, the second is not examined. If neither meets the requirement, the candidate sequence is not scored. In order to determine whether...

example 4

[0452] Isolation of cDNA Clones Encoding Human PRO Polypeptides

[0453] Using the techniques described in Examples 1 to 3 above, numerous full-length cDNA clones were identified as encoding PRO polypeptides as disclosed herein. These cDNAs were then deposited under the terms of the Budapest Treaty with the American Type Culture Collection, 10801 University Blvd., Manassas, Va. 20110-2209, USA (ATCC) as shown in Tabl 7 below.

8 TABLE 7 Material ATCC Dep. No. Deposit Date DNA26843-1389 203099 Aug. 4,1998 DNA30867-1335 209807 Apr. 28, 1998 DNA34431-1177 209399 Oct. 17, 1997 DNA38268-1188 209421 Oct. 28, 1997 DNA40621-1440 209922 Jun. 2, 1998 DNA40625-1189 209788 Apr. 21, 1998 DNA45409-2511 203579 Jan. 12,1999 DNA45495-1550 203156 Aug. 25, 1998 DNA49820-1427 209932 Jun. 2, 1998 DNA56406-1704 203478 Nov. 17, 1998 DNA56410-1414 209923 Jun. 2, 1998 DNA56436-1448 209902 May 27, 1998 DNA56855-1447 203004 Jun. 23, 1998 DNA56860-1510 209952 Jun. 9, 1998 DNA56862-1343 203174 Sep. 1, 1998 DNA56868-...

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Abstract

The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

Description

[0001] This is a continuation application claiming priority under 35 USC .sctn.120 to U.S. Ser. No. 10 / 006867 Filed Dec. 6, 2001, and which claims priority under 35 USC .sctn.119 to U.S. provisional serial Nos. 60 / 063,435 Filed Nov. 29, 1997; 60 / 064,215 Filed Nov. 29, 1997; 60 / 082,797 Filed Apr. 22, 1998; 60 / 083,495 Filed Apr. 29, 1998; 60 / 085,579 Filed May 15, 1998; 60 / 087,759 Filed Jun. 2, 1998; 60 / 088,021 Filed Jun. 2, 1998; 60 / 088,029 Filed Jun. 4, 1998; 60 / 088,030 Filed Jun. 4, 1998; 60 / 088,734 Filed Jun. 10, 1998; 60 / 088,740 Filed Jun. 10, 1998; 60 / 088,811 Filed Jun. 10, 1998; 60 / 088,824 Filed Jun. 10, 1998; 60 / 088,825 Filed Jun. 10, 1998; 60 / 088,863 Filed Jun. 11, 1998; 60 / 089,105 Filed Jun. 12, 1998; 60 / 089,514 Filed Jun. 16, 1998; 60 / 089,653 Filed Jun. 17, 1998; 60 / 089,952 Filed Jun. 19, 1998; 60 / 090,246 Filed Jun. 22, 1998; 60 / 090,444 Filed Jun. 24, 1998; 60 / 090,688 Filed Jun. 25, 1998; 60 / 090,696 Filed Jun. 25, 1998; 60 / 090,862 Filed Jun. 26, 1998; 60 / 091,628 Filed Jul. 2...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K38/17C07H21/04C07K1/00C07K14/00C07K14/435C07K14/47C07K16/18C07K16/28C07K16/30C07K16/40C07K17/00C12N1/21C12N5/06C12N9/00C12P21/02G01N33/53
CPCC07K14/47C07K16/3053C07K16/18A61K38/00C07K14/705C07K16/3023C07K16/3046C07K2319/30Y10S530/866C07K14/82C07K16/30C07K14/4748C07K16/40C12N5/06C07K16/3038C07K14/52C12N9/6424C12P21/02
Inventor EATON, DAN L.FILVAROFF, ELLENGERRITISEN, MARY E.GODDARD, AUDREYGODOWSKI, PAUL J.GRIMALDI, J. CHRISTOPHERGURNEY, AUSTIN L.WATANABE, COLIN K.WOOD, WILLIAM I.
Owner GENENTECH INC