Detection of nucleic acid hybrids

Inactive Publication Date: 2005-09-29
PROMEGA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0141] An advantage of the invention is that the presence or absence of one or more target nucleic acid(s) can be detected reliably, reproducibly, and with great sensitivity.
[0142] A further benefit of the invention is that quantitative information can be obtained about the amount of a target nucleic acid sequence in a sample.
[0143] A further advantage of the invention is that very slight differences in nucleic acid sequence are detectable, including si

Problems solved by technology

PCR-based methods are of limited use for the detection of nucleic acid of unknown sequence.
The throughput of analysis by this technique is limited because samples require processing, gel analysis, and significant interpretation of data before SNPs can be accurately determined.
In addition to the limited throughput possible by gel-based techniques, the design and interpretation of SSCP based experiments can be di

Method used

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  • Detection of nucleic acid hybrids
  • Detection of nucleic acid hybrids
  • Detection of nucleic acid hybrids

Examples

Experimental program
Comparison scheme
Effect test

Example

EXAMPLE 1

Comparison of Signal Strengths During Allele Determination Using Probes that Interrogate the Same DNA Strand Versus Probes that Interrogate Different Strands

[0455] Because DNA normally exists in a eukaryotic genome as a double-stranded polymer; in theory, allele discrimination could be performed by:

[0456] A) using probes that are essentially identical in sequence (except for an allele discriminating base) and that hybridize to the same DNA strand; or

[0457] B) using two probes that hybridize to different strands of DNA but match the sequence of only one allele of the gene at a position where the genotype is to be determined.

[0458] In this example, a comparison is made of the signal strengths of these two types of probes in distinguishing a single nucleotide polymorphism (SNP) target nucleic acid provided as a homozygous target for any known allele or as a heterozygous target containing different alleles.

[0459] Oligonucleotide PH1 (SEQ ID NO:1) is a probe that encodes a...

Example

EXAMPLE 2

Reduction of Probe-Alone Background Values for Probes Designed to Interrogate a Viral Sequence

[0468] In this example, the background light values from probe-alone reactions are reduced by alteration of reaction conditions. More specifically, the values from such background reactions are reduced by lowering the Klenow exo− level in the reactions as shown in Example 43. In addition, the probes are used to assay the relative probe signal strength values for probes that hybridize to the same DNA strand versus probes that hybridize to different strands but that interrogate the same nucleotide polymorphism site.

[0469] Oligonucleotides CV11 (SEQ ID NO:8) and CV12 (SEQ ID NO:9) are a pair of single-stranded DNAs that can hybridize together to produce a segment of the genome of cytomegalovirus (CMV) in a form sensitive to the drug gancyclovir. Oligonucleotides CV13 (SEQ ID NO:10) and CV14 (SEQ ID NO:11) are a pair of single-stranded DNAs that can hybridize together to produce the...

Example

EXAMPLE 3

Multiplex Analysis of Alleles at One Interrogation Site

[0479] For a wide variety of genetic disorders, only a very small percentage of samples will have a particular single nucleotide polymorphism (SNP) at any one site. For this reason, it can be much more efficient in these cases to screen for the presence of groups of mutant alleles and to perform secondary, single probe tests only if there is a positive signal for any of the probes designed to detect the mutant sites. Such a form of multiplex analysis will be performed in this example.

[0480] Multiple probes designed to detect a mutant form of a gene in the CMV genome are used in one reaction and the signal from this reaction is compared to that from a probe that is specific for the non-mutated sequence. In this example, the SNP sites are separated by only one base and the alleles are provided as pure nucleic acid target species.

[0481] Oligonucleotides CV19 (SEQ ID NO:16) and CV20 (SEQ ID NO:17) encode a segment of th...

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Abstract

Processes are disclosed using the depolymerization of a nucleic acid hybrid to qualitatively and quantitatively analyze for the presence of a predetermined nucleic acid. Applications of those processes include the detection of single nucleotide polymorphisms, identification of single base changes, speciation, determination of viral load, genotyping, medical marker diagnostics, and the like.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation-in-part of U.S. Ser. No. 09 / 252,436, filed on Feb. 18, 1999, which is a continuation-in-part of U.S. Ser. No. 09 / 042,287, filed Mar. 13, 1998, both of which are incorporated herein by reference.FIELD OF THE INVENTION [0002] The invention relates to nucleic acid detection. More specifically, the invention relates to the detection of targeted, predetermined nucleic acid sequences in nucleic acid target hybrids, and the various applications of their detection. BACKGROUND OF THE INVENTION [0003] Methods to detect nucleic acids and to detect specific nucleic acids provide a foundation upon which the large and rapidly growing field of molecular biology is built. There is constant need for alternative methods and products. The reasons for selecting one method over another are varied, and include a desire to avoid radioactive materials, the lack of a license to use a technique, the cost or availability of reag...

Claims

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Application Information

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IPC IPC(8): G01N33/53C12N1/15C12N1/19C12N1/21C12N5/10C12N9/12C12N15/09C12N15/54C12Q1/04C12Q1/48C12Q1/66C12Q1/68C12R1/01G01N33/566
CPCC12Q1/04C12Q1/66C12Q1/68C12Q1/6823C12Q1/6827Y10T436/24C12Q2535/131C12Q2521/319C12Q2535/107C12Q2565/301C12Q2565/1015C12Q2537/101C12Q2565/627
Inventor SHULTZ, JOHNLEWIS, MARTINLEIPPE, DONNAMANDREKAR, MICHELLEKEPHART, DANIELRHODES, RICHARDANDREWS, CHRISTINEHARTNETT, JAMESGU, TRENTOLSON, RYANWOOD, KEITHWELCH, ROY
Owner PROMEGA
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