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Mammalian cytokines; related reagents and methods

a technology of cytokines and cytokines, applied in the field of proteins, can solve the problems of hampered research to better understand and treat various immune disorders

Inactive Publication Date: 2007-03-01
SCHERING CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0024] The present invention is directed to mammalian, e.g., rodent, canine, feline, primate, interleukin-B50 (IL-B50) and its biological activities. It includes nucleic acids coding for polypeptides themselves and methods for their production and use. The nucleic acids of the invention are characterized, in part, by their homology to cloned complementary DNA (cDNA) sequences enclosed herein, and/or by functional assays for growth factor- or cytokine-like activities, e.g., IL-7 (see Maeurer, e

Problems solved by technology

Research to better understand and treat various immune disorders has been hampered by the general inability to maintain cells of the immune system in vitro.

Method used

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  • Mammalian cytokines; related reagents and methods
  • Mammalian cytokines; related reagents and methods
  • Mammalian cytokines; related reagents and methods

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I. General Methods

[0143] Many of the standard methods below are described or referenced, e.g., in Maniatis, et al. (1982) Molecular Cloning, A Laboratory Manual Cold Spring Harbor Laboratory, Cold Spring Harbor Press, NY; Sambrook, et al. (1989) Molecular Cloning: A Laboratory Manual (2d ed.) Vols. 1-3, CSH Press, NY; Ausubel, et al., Biology Greene Publishing Associates, Brooklyn, N.Y.; or Ausubel, et al. (1987 and Supplements) Current Protocols in Molecular Biology Wiley / Greene, NY; Innis, et al. (eds. 1990) PCR Protocols: A Guide to Methods and Applications Academic Press, NY. Methods for protein purification include such methods as ammonium sulfate precipitation, column chromatography, electrophoresis, centrifugation, crystallization, and others. See, e.g., Ausubel, et al. (1987 and periodic supplements); Deutscher (1990) “Guide to Protein Purification,”Methods in Enzymology vol. 182, and other volumes in this series; Coligan, et al. (1995 and supplements) Current Protocols in...

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Abstract

Purified genes encoding cytokines from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided.

Description

[0001] This filing is a Continuation-in-Part of U.S. Patent Application U.S. Ser. No. 09 / 399,492, filed Sep. 20, 1999, and claims benefit of U.S. Provisional Patent Applications U.S. Ser. No. 60 / 131,298, filed Apr. 27, 1999, and U.S. Ser. No. 60 / 101,318, filed Sep. 21, 1998, all of which are incorporated herein by reference.FIELD OF THE INVENTION [0002] The present invention pertains to compositions related to proteins which function in controlling biology and physiology of mammalian cells, e.g., cells of a mammalian immune system. In particular, it provides purified genes, proteins, antibodies, and related reagents useful, e.g., to regulate activation, development, differentiation, and function of various cell types, including hematopoietic cells. BACKGROUND OF THE INVENTION [0003] Recombinant DNA technology refers generally to the technique of integrating genetic information from a donor source into vectors for subsequent processing, such as through introduction into a host, where...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C07H21/04C12P21/02C07K14/52C07K14/54
CPCC07K14/54
Inventor BAZAN, J. FERNANDODE WAAL MALEFYT, RENELIU, YONG-JUNSOUMELIS, VASSILI
Owner SCHERING CORP