Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Canola extracts containing high levels of phenolic acids

a technology of phenolic acids and canola, which is applied in the direction of biocide, drug composition, metabolic disorder, etc., can solve the problems of anticancer agents having adverse effects on the immune system of patients, their potential toxic effects, etc., and achieve the effect of preventing or minimizing sunburn

Inactive Publication Date: 2007-05-03
KGK SYNERGIZE INC
View PDF2 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0026] It is an object of certain embodiments of the present invention to provide methods of preventing or minimizing sunburn comprising topically administering a sunscreen formulation comprising a canola extract.
[0036] Certain embodiments of the present invention are directed to a method of preventing or minimizing sunburn comprising topically administering an effective amount of a topical sunscreen formulation disclosed herein.

Problems solved by technology

However the fact that they do harm normal cells indicates their potential for toxicity.
However, some anticancer agents have adverse effects on the patients immune system.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Canola extracts containing high levels of phenolic acids
  • Canola extracts containing high levels of phenolic acids
  • Canola extracts containing high levels of phenolic acids

Examples

Experimental program
Comparison scheme
Effect test

example 1

Extraction of CPA (Canola Phenolic Acid) From Canola Meal

[0088] The extract enriched in total phenolics was isolated from canola meal by homogenization in methanol: water, 70:30, v / v and evaporation of supernatant to dryness. According to HPLC analysis (R. Amarowicz), this extract (Phenolic Extract Saskatoon Research Centre, Agriculture and Agri-Food Centre, Apr. 24, 2002) contained 5.3% phenolic acids (Table 1).

TABLE 1Composition of phenolic acids in theextract from canola meal (mg / g)p-Proto-SinapicFeruliccoumaricCaffeiccatechuicEsters50.750.560.080.07TraceFree1.030.020.020.03traceInsoluble-0.520.010.06Trace—boundTotal53.15 mg / g (5.3%)

[0089] This extract from canola meal had a substantial anti-proliferative activity against human skin cancer cell line SK-MEL5 in the Alamar Blue assay. The bioactive fraction was subsequently isolated by a large-scale procedure (developed for that purpose), tested again for anti-proliferative activity in SK-MEL5 cells. IC50 concentrations (concen...

example 2

In Vitro Analysis

[0091] To evaluate anticancer activities of CPA, B16-F1 and B16-F10 malignant melanoma tumor cells (10,000) were cultured with CPA (solubilized in dimethyl sulfoxide, DMSO)) at 160, 80, 40 and 20 μg / ml. The cells were incubated for 72 hours in culture in presence or absence of extracts. The cells were cultured at 37° C. in 5% CO2 for 48 and 72 hours. [3H] thymidine was added to the cell cultures, cell proliferation was measured by [3H] thymidine uptake using Micro beta-Scintillation counter. See FIGS. 2A, 2B, 3A and 3B.

[0092] Preliminary in vitro studies showed that CPA inhibited the proliferation of B16-F1 and B16-F10 malignant melanoma tumor cells for up to 72 hours in culture in a dose dependent manner.

example 3

In Vivo Analysis

[0093] Male C57BL / 6 mice (17 wks old) were acclimatized for 4 days. Mice were treated for a week (oral) with CPA followed by injections of B16-F1 malignant melanoma tumor cells (2.0×105) in 100 μl saline subcutaneously in the right hind flank of mice. Mice were monitored for tumor growth for 28 days from the date of injection.

a) Effect of CPA Extracts on Development of Melanoma in Mice (Oral Gavage).

[0094] Mice were given 6.0% CPA w / v of CPA in Canola oil (0.2 ml) by oral gavages daily for additional 4 weeks after tumor cell injection.

[0095] Tumor Volume: When primary tumours became visible / palpable, their maximum length and width were measured and tumour volume calculated (p<0.04). It was observed that the growth of tumor was similar in mice treated with CPA by oral gavage as compared to the controls up to day 18 of treatment. After day 18, CPA treated group showed a significant reduction in the tumor volume as compared to control suggesting inhibition of tumo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Fractionaaaaaaaaaa
Login to View More

Abstract

Disclosed in certain embodiments is a canola extract comprising greater than 30% sinapic acid, pharmaceutical compositions thereof, and methods thereof.

Description

BACKGROUND [0001] Skin cancer is the most commonly occurring cancer, accounting for 40% of all new cancer diagnoses and for about 2% of all cancer deaths (1). It is a disease in which cancer (malignant) cells are found in the outer layers of the skin. [0002] There are three main types of skin cancer: [0003] a) Non-melanoma—common types such as basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). [0004] b) Melanoma—rare type, found in the cells that color the skin (melanocytes). [0005] c) Actinic keratosis, pre-malignant skin lesion, often progresses to squamous cell cancer. [0006] The incidence of melanoma and non-melanoma skin cancer appears to be increasing (2, 3), although melanoma incidence rates may have stabilized in the 1990s (4). Epidemiological evidence suggests that aging population, exposure to ultraviolet (UV) radiation and the sensitivity of an individual's skin to UV radiation are risk factors for skin cancer, although the type of exposure (high-intensity inte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/31
CPCA61K36/31A61K31/192A61P3/06A61P35/00A61K9/0014A61K9/0053A61K36/00
Inventor GUTHRIE, NAJLAGUTHRIE, ROBERT A.
Owner KGK SYNERGIZE INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com