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Device and method of maintaining sperm motility in a capillary-loaded chamber

a technology of capillary-loaded chambers and sperm motility, which is applied in the direction of instruments, analysis using chemical indicators, manufacturing tools, etc., can solve the problems of increasing the chamber depth, affecting the operation of the device, and affecting the effect of sperm motility

Inactive Publication Date: 2007-05-03
HAMILTON THORNE BIOSCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A defect of the radially-loaded chamber is that the coverslip is not constrained and can therefore rise up, increasing the chamber depth.
A particle only one micron in diameter on the posts will cause an error of 10% in the concentration calculation from the radially-loaded chamber.
Moreover, after use, the chamber has to be cleaned, and its operation is consequently slower and the resulting concentration calculation is less accurate than the disposable capillary-loaded chambers.
Possible causes include the greater chamber depth, potential toxic effects in the fixed-coverslip chambers, and possible damage to sperm during the capillary inflow loading phase.

Method used

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  • Device and method of maintaining sperm motility in a capillary-loaded chamber
  • Device and method of maintaining sperm motility in a capillary-loaded chamber
  • Device and method of maintaining sperm motility in a capillary-loaded chamber

Examples

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Effect test

example 1

Effect of Correction on Motility

[0020] The motion parameters of a thawed bovine sperm sample were measured in an IVOS analyzer, with 1.75× multiplier, giving a net magnification of 17.5×. All slides had corrected smoothed coverslips and were maintained at 37° C. Fired coverslips, in which the sharp edges were removed by melting in a butane flame instead of by grinding, were also used for drop-loading and for capillary loading, as described below. Progressive motility, which is the fraction of cells with average path velocity (VAP) >75 μm / sec and path straightness (STR) >50%, and the standard deviations are shown in FIG. 3 for four types of loading methods described below.

[0021] Drop Load Method:

[0022] 23 μl of sample were dropped on a microscope slide, not loaded by capillary action, and a fired coverslip was placed on it, with 20 μm spacers to maintain 20 μm chamber depth. The fired coverslip had been heated in a flame to melt the glass edge. The slides and coverslips were the s...

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Abstract

The invention relates, generally, to a method of removing sharp edges from a microscope coverslip comprising grinding down the edges and polishing the edges The invention also relates to a device for determining cell motility comprising a slide, a coverslip, comprising at least one edge that has been smoothed and a chamber, created by the slide and the coverslip and which is tangential to the coverslip, such that motile cells entering the chamber are substantially undamaged. The invention also relates to a method for using the device to determine cell motility.

Description

[0001] The present application claims priority to Provisional Application Ser. No. 60 / 719,392 filed on Sep. 22, 2005.INTRODUCTION [0002] The invention relates, generally, to a method of removing sharp edges from a microscope coverslip comprising grinding down the edges and polishing the edges. The invention also relates to a device for determining cell motility comprising a slide, a coverslip, comprising at least one edge that has been smoothed and a chamber, created by the slide and the coverslip and which is tangential to the coverslip, such that motile cells entering the chamber are substantially undamaged. The invention also relates to a method for using the device to determine cell motility. BACKGROUND OF INVENTION [0003] Measurement of sperm motility (fraction of motile cells) is of considerable importance in the animal breeding market. The reason is that motility is a measure of the animals breeding effectiveness, since only motile sperm can fertilize the ovum. For a given nu...

Claims

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Application Information

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IPC IPC(8): C12Q1/02C12M1/34
CPCB24B9/08B24B9/20C12Q1/025G02B21/34
Inventor DOUGLAS-HAMILTON, DIARMAID H.HAMILTON, MEG D.
Owner HAMILTON THORNE BIOSCI
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