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Composition for skin external application containing gallocatechin gallate for moisturizing effect on the skin

Inactive Publication Date: 2009-07-09
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0007]Accordingly, the present invention has been made to solve the above-mentioned problems occurring in the prior art. The inventors of the present invention have many studies to search for the components that shows a skin-moisturizing effect by controlling activity of the peroxisome proliferator activated receptor isoform alpha (PPAR-α), among various factors affecting the skin in terms of skin moisturization and skin protection. Then, we have found that when a composition for external skin application comprises gallocatechin gallate contained in green tea leaves, it activates the peroxisome proliferator activated receptor isoform alpha (PPAR-α) and stimulates expression of skin moisturizing factors such as filaggrin and involucrin, and thus provides excellent skin moisturizing and anti-drying effects. Particularly, we have also found that when the composition further comprises theobromine and quercetin, besides gallocatechin galate, at an optimized ratio, the above effects can be maximized. The present invention is based on these findings.
[0008]Therefore, it is an object of the present invention to provide a composition for external skin application having a skin-moisturizing effect, which comprises gallocatechin gallate as an active ingredient.

Problems solved by technology

However, skin drying and roughening phenomena, caused by a drop in moisture content of the stratum corneum and including loose, dry and inanimate skin conditions, occur due to various causes.
However, such humectants have a disadvantage in that they have a highly sticky and dense feel when applied on the skin.
However, such occlusive moisturizers have difficulty in maintaining stability of an emulsified formulation and are not amenable to production of transparent gel-like cosmetic products.
However, in the relevant art, there is no disclosure of a skin cosmetic agent utilizing a mechanism of controlling PPAR activities other than a cosmetic composition (WO01 / 008653) for preventing and treating skin aging.

Method used

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  • Composition for skin external application containing gallocatechin gallate for moisturizing effect on the skin
  • Composition for skin external application containing gallocatechin gallate for moisturizing effect on the skin
  • Composition for skin external application containing gallocatechin gallate for moisturizing effect on the skin

Examples

Experimental program
Comparison scheme
Effect test

experimental example 1

Determination of PPAR-α Activation Capability Using One-Factor-At-a-Time (OFAT) Experiment

[0026]The following test was carried out to determine PPAR-A activation capability.

[0027]CV-1 cells (ATCC CCL 70), i.e. monkey kidney epithelial cell line, were subcultured in a DMEM medium containing 10% bovine fetal serum treated with charcoal / dextrin. A phenol red-free medium was used to avoid the effect of estrogen upon phenol red. As plasmids, used were plasmids having PPRE (PPARs responsive element) as a promoter, followed by firefly luciferase genes as a reporter, the PPRE being activated by PPAR-(gene-containing PPAR- and ligand-bound PPAR-) bound next to the universal promoter expressed under general culture conditions, and a reference plasmid to which renilla luciferase genes were bound.

[0028]CV-1 cells were plated on a 24-well microtiter plate at a concentration of 5×104 cells per well and cultured for 24 hours. Then, the above three types of plasmid genes were subjected to transient...

experimental example 2

Determination of PPAR-α Activation Capability Using Response Surface Methodology (RSM)

[0030]To optimize the PPAR-α activation capability of the components contained in green tea leaves, i.e. gallocatechin gallate, theobromine and quercetin, PPAR-α activation capability of which was determined in Experimental Example 1, response surface methodology (RSM) was used. When using RSM, it is possible to understand the level of an independent parameter where a response value is optimized, to estimate the effect of an independent parameter upon response parameters via estimation of a functional relationship between each independent parameter and dependent parameter, and to determine optimized experimental conditions. According to the present invention, central composite designs of RSM were carried out by using MiniTab 14. The results are shown in the following Table 2.

TABLE 2Central Composite Designs and Measurements ThereofC5C6C7C8C9C10C11C12↓GCGtheobrominequercetinStdOrder_1RunOrder_1Block...

example 3

Determination of Expression of Filaggrin via RT-PCR Analysis

[0036]The cell line used in this example was a human keratinocyte HaCaT cell line distributed from Dr. Fusening in the German Cancer Research Center. The cells were pipetted into 60 mm dishes in a number of 1×105 cells per dish, cultured for one day, and treated with each test sample, followed by culturing for 24 hours. The test samples include a negative control (lanes 1 and 5), a mixture of gallocatechin gallate:theobromine:quercetin (130 μM:100 μM:100 μM) (lanes 2 and 6), and a mixture of gallocatechin gallate:theobromine:quercetin (65 μM:50 μM:50 μM) (lanes 3 and 7). The cells were cultured in a DMEM (Dulbeco's modified eagles medium, GibcoBRL, Life Technology) containing 10% fetal bovine serum (FBS) at 37° C. under 5% CO2. The negative control was the cells cultured in the same medium in the absence of any test sample.

[0037]From the cells cultured and tested in the manner as described above, the total RNA was extracted...

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Abstract

The present invention relates to a composition for external skin application having a skin-moisturizing effect, which comprises gallocatechin gallate as an active ingredient. More particularly, the composition for external skin application comprises gallocatechin gallate as an active ingredient to activate peroxisome proliferator activated receptor isoform alpha (PPAR-α), to stimulate expression of filaggrin and involucrin that are skin-moisturizing factors, and thus to provide excellent anti-drying and skin-moisturizing effects. More particularly, the composition for external skin application may further comprise theobromine and quercetin in addition to gallocatechin gallate to maximize such effects.

Description

TECHNICAL FIELD[0001]The present invention relates to a composition for external skin application having a skin-moisturizing effect, which comprises gallocatechin gallate as an active ingredient. More particularly, the composition for external skin application according to the present invention comprises gallocatechin gallate as an active ingredient to activate peroxisome proliferator activated receptor isoform alpha (PPAR-α), to stimulate expression of filaggrin and involucrin that are skin-moisturizing factors, and thus to provide excellent anti-drying and skin-moisturizing effects. More particularly, the composition for external skin application according to the present invention may further comprise theobromine and quercetin in addition to gallocatechin gallate to maximize such effects.BACKGROUND ART[0002]In general, the skin is divided into the epidermis, the dermis and the subcutaneous tissue when viewed from the exterior, and functions to protect the whole organs in the body ...

Claims

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Application Information

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IPC IPC(8): A61K31/522A61K31/353A61P17/00
CPCA61Q19/007A61K8/498A61P17/00A61K8/49A61Q19/00
Inventor SHIN, HYUN JUNGKIM, JEONG KIKIM, SU NAMLEE, SANG MINLEE, BYEONG GONCHANG, IH SEOP
Owner AMOREPACIFIC CORP
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