Anti-aging composition for vitalization of retinoic acid receptor that contains mixed extract of chinese matrimony vine and pine needles as effective component
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example 1
Preparation of Lycium chinense Fruit Extract
[0019]Lycium chinense fruits were washed with purified water, washed and finely powdered. 200 g of the Lycium chinense fruit powder was added to 1 liter of an aqueous solution of 70% ethanol and extracted by heating for 12 hours in an extractor equipped with a cooling condenser. The extract was filtered through 300-mesh filter cloth, and the filtrate was allowed to stand at 4˜15° C. for 7 days, and then filtered through Whatman No. 2 filter paper.
[0020]The filtered extract was placed in a 3-liter separatory funnel, and 1 liter of ethyl acetate was added thereto. Then, the solution was mixed well by shaking. After the solution was completely separated into two layers, the upper layer (ethyl acetate layer) was collected. The lower layer (aqueous layer) was extracted twice more using a separatory funnel.
[0021]The separated upper layers were combined with each other and concentrated under reduced pressure at 50° C. using a distillation apparat...
example 2
Preparation of Pine Needle Extract
[0022]Pine needles were washed with purified water, washed and finely powdered. 200 g of the pine needle powder was added to 1 liter of an aqueous solution of 70% ethanol and extracted by heating for 12 hours in an extractor equipped with a cooling condenser. The extract was filtered through 300-mesh filter cloth, and the filtrate was allowed to stand at 4˜15° C. for 7 days, and then filtered through Whatman No. 2 filter paper.
[0023]The filtered extract was placed in a 3-liter separatory funnel, and 1 liter of ethyl acetate was added thereto. Then, the solution was mixed well by shaking. After the solution was completely separated into two layers, the upper layer (ethyl acetate layer) was collected. The lower layer (aqueous layer) was extracted twice more using a separatory funnel.
[0024]The separated upper layers were combined with each other and concentrated under reduced pressure at 50° C. using a distillation apparatus equipped with a cooling con...
formulation example 1
Milk Lotion
[0035]According to the composition shown in Table 3 below, milk lotion can be prepared by a conventional method.
TABLE 3ComponentsContent (wt %)Glycerin8.0Butylene glycol4.0Hyaluronic acid extract5.0Beta-glucan7.0Carbomer0.1Mixture of Lycium chinense extract and1.0pine needle extractCaprylic / capric triglyceride8.0Squalane5.0Cetearyl glucoside1.5Sorbitan stearate0.4Cetearyl alcohol1.0Preservativeq.s.Fragranceq.s.Pigmentq.s.Triethanoamine0.1Purified waterBalance
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