Release reagent for vitamin d compounds

Abstract

A reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein and an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition as well as the reagent mixture obtained in this manner. Also disclosed is the use of the reagent compositions to release vitamin D compounds as well as a kit for detecting a vitamin D compound.

Description

BACKGROUND INFORMATION[0001]The present invention concerns a reagent composition for releasing vitamin D compounds bound to vitamin D-binding protein, an in vitro method for the detection of a vitamin D compound in which the vitamin D compound is released from vitamin D-binding protein by the use of this reagent composition and the reagent mixture obtained in this manner. It also concerns the use of the disclosed reagent composition to release vitamin D compounds as well as a kit for detecting a vitamin D compound which contains the reagent composition for releasing vitamin D compounds in addition to common detecting reagents.[0002]An adequate supply of vitamin D is vital as the term “vitamin” already suggests. A deficiency of vitamin D leads to severe diseases such as rickets or osteoporosis. While vitamin D was still regarded as a single substance at the beginning of the last century, the vitamin D system has changed in the course of the last decades into a complex and manifold ne...

AI Technical Summary

Benefits of technology

The patent text describes the use of a reagent composition to detect vitamin D compounds. The reagent composition includes a hydrogen carbonate salt and a substance that releases hydrogen carbonate ions upon hydrolysis. The presence of these components causes a pH shift during the pre-treatment reaction, which can lead to denaturation of vitamin D-binding protein and facilitate detection of a vitamin D compound. After the pre-treatment step, unspecific proteins can be added to block the residual reducing capability of the reducing agent, allowing for the noncompromised detection of a vitamin D compound.

Problems solved by technology

Measuring the vitamin D level itself is of little benefit when determining the vitamin D status of a patient, because concentrations of vitamin D (vitamin D2 and vitamin D3) fluctuate greatly depending on food uptake or exposure to sunlight.

In addition vitamin D has a relatively short biological half-life in the circulation (24 hours) and it is therefore also for this reason not a suitable parameter for determining the vitamin D status of a patient.

The binding of 25-hydroxyvitamin D or other vitamin D compounds to the vitamin D-binding protein enormously complicates the determination of vitamin D compounds.

Despite immense efforts in recent years, all available methods for determining vitamin D have disadvantages such as laborious sample preparation, poor standardization, poor agreement between test procedures or bad recovery of spiked vitamin D (see for this in particular Zerwekh, J. E., supra).

It is particularly difficult to automate a test for a vitamin D compound.

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