Method for Obtaining Sterile Human Amniotic Fluid and Uses Thereof

Inactive Publication Date: 2014-11-13
MAM HLDG OF WEST FLORIDA L L C
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0009]The present invention has been made keeping in mind the above problems occurring in the prior art, and an object of the present invention is to provide an apparatus for collecting amniotic fluid from the uterus, which can not only significantly decrease the risk occurring when a needle is directly inserted into the uterus

Problems solved by technology

The insertion of the needle, however, entails the risk of complications and medical accidents, causing anxiety and pain to the pregnant woman.
While amniotic fluid cells can be obtained from a small amount of fluid during amniocentesis, these amounts are insufficient for a larger scale harvestin

Method used

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  • Method for Obtaining Sterile Human Amniotic Fluid and Uses Thereof
  • Method for Obtaining Sterile Human Amniotic Fluid and Uses Thereof
  • Method for Obtaining Sterile Human Amniotic Fluid and Uses Thereof

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Embodiment Construction

[0019]As used herein in the specification, “a” or “an” may mean one or more. As used herein in the claim(s), when used in conjunction with the word “comprising”, the words “a” or “an” may mean one or more than one.

[0020]As used herein “another” or “other” may mean at least a second or more of the same or different claim element or components thereof. Similarly, the word “or” is intended to include “and” unless the context clearly indicates otherwise. “Comprise” means “include.”

[0021]In one embodiment of the present invention there is provided a method of obtaining sterile human amniotic fluid from an individual, comprising the steps of inserting a blunt tip needle into the amniotic sac of said individual; attaching the blunt tip needle to a three-way stopcock; connecting a Luer lock syringe to the three-way stopcock; connecting a first end of a length of sterile tubing with the three-way stopcock; and collecting sterilely said amniotic fluid through the blunt tip needle and sterile ...

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Abstract

Provided herein is a pre-Caesarean method for collecting amniotic fluid from a patient. A needle is inserted into the incision site for the future C-section, which may be under ultrasound guidance, through which the amniotic fluid is drawn under a low level suction and, optionally, gravity to a sterile collection container. The method encompasses filtering and/or irradiating the amniotic fluid to collect biomolecules of interest such as growth factors and/or stem cells. Also provided is the sterile amniotic fluid or filtrates thereof collected by the method described herein

Description

CROSS-REFERENCE TO RELATED APPLICATION[0001]This nonprovisional application claims benefit of priority under 35 U.S.C. §119(e) of provisional application U.S. Ser. No. 61 / 821,868, filed May 10, 2013, now abandoned, the entirety of which is hereby incorporated by reference.BACKGROUND OF THE INVENTION[0002]1. Field of the Invention[0003]The present invention relates generally to the fields of the sterile obtention of a biological fluid and to the isolation and culture of biomolecules and cells from a biological fluid. More specifically, the present invention relates to a pre-cesarian surgical method for the sterile collection and filtering of amniotic fluid for the isolation of biomolecules and stem cells therefrom.[0004]2. Description of the Related Art[0005]Within the uterus of a pregnant woman, a growing fetus is surrounded and cushioned by amniotic fluid, a watery liquid within the amnion. Amniotic fluid is one of the main samples used for the medical examination of the pregnant w...

Claims

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Application Information

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IPC IPC(8): A61M1/00C12N5/073
CPCA61M1/0056A61M1/0039C12N5/0605A61B10/0048A61M2202/0437A61M2202/0494A61M2205/075A61M1/82A61M1/682A61M1/604
Inventor HARRELL, CARL RANDALL
Owner MAM HLDG OF WEST FLORIDA L L C
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