In vitro method for detecting intestinal barrier failure in animals by determining ovotransferrin

Abstract

The present invention pertains to an in vitro method for detecting intestinal barrier failure in animals, the method comprising the following steps: a) collecting intestinal sample material of an individual animal or of an animal population; and b) determining the amount of at least one protein marker contained in said sample material; wherein the at least one protein marker comprises or consists of ovotransferrin or a functional fragment thereof, and wherein an increased amount of said at least one protein marker contained in said sample versus a reference sample indicates intestinal barrier failure.

Description

FIELD OF THE INVENTION[0001]The present invention relates to an in vitro method for detecting intestinal barrier failure in animals. More specifically, the present invention pertains to an acute phase protein (APP)-based method for evaluating the gut health status of an individual animal and of an animal population, respectively.BACKGROUND OF THE INVENTION[0002]Intestinal health is critically important for the welfare and performance of livestock animals. Enteric diseases that affect the structural integrity of the gastrointestinal tract (GIT) lead to high economic losses due to reduced weight gain, poor feed conversion efficiency, increased mortality rates and greater medication costs (M'Sadeq, S. A., Wu, S., Swick, R. A. & Choct, M. (2015). Towards the control of necrotic enteritis in broiler chickens with in-feed antibiotics phasing-out worldwide. Animal Nutrition, 1, 1-11; Timbermont, L., Haesebrouck, F., Ducatelle, R. & Van Immerseel, F. (2011). Necrotic enteritis in broilers: ...

AI Technical Summary

Benefits of technology

This method provides a fast, reliable, and non-invasive means to detect intestinal barrier failure in individual animals or populations, enabling early intervention to improve gut health and prevent economic losses due to enteric diseases.

Problems solved by technology

Enteric diseases that affect the structural integrity of the gastrointestinal tract (GIT) lead to high economic losses due to reduced weight gain, poor feed conversion efficiency, increased mortality rates and greater medication costs (M'Sadeq, S. A., Wu, S., Swick, R. A. & Choct, M.

inical form. Acute NE typically leads to a massive increase in flo

h performance. The reduction in performance is not only associated with impaired growth rate and feed conversion during production, but also with increased condemnation rates in broilers due to hepatitis at proces

In addition, intestinal barrier failure might enable normal inhabitants of the GIT, like Enterococcus caecorum to invade the systemic circulation.

This can lead to further diseases like arthritis and osteomyelitis and finally lead to lower performance of the animals or the animal flock, respectively.

However, none of the proposed strategies represent a good marker for the broiler industry as they are either not applicable under field conditions (e.g. oral administration of a marker that can be measured in the blood on a later timepoint (Gilani, S., Howarth, G. S., Kitessa, S. M., Tran, C. D., Forder, R. E. A. & Hughes, R. J. (2017).